Synthesis Of Poly P Xylylenes
High resolution structure of the ba3 cytochrome c oxidase from Thermus thermophilus in a lipidic environment.
... (27 lipids per protein trimer covering 79% of the trimer hydrophobic surface, PDB ID 1QHJ ), a potassium channel crystallized in a lipid/ detergent mixture (64 lipids per protein tetramer, PDB ID 2R9R ), and two dimensional crystals of aquaporin AQP0 (36 lipids per protein tetramer, PDB ID 2B6O ). The new highresolution structure of ba3-oxidase ... to lipids or detergents observed in one or more aa3-structures. OLC16 and OLC3 occupy similar positions to the two alkyl chains of the phospholipid PGV525 in 2DYR. The alkyl chain of OLC3 occupies a very similar position to the alkyl chain of the detergent LMT572 of 3HB3, while a portion of the detergent head group overlaps with the head groups of both OLC3 ... a phospholipid tail of PSC in 2DYR. Interestingly, this site does not exist in the structure of ba3: instead, the N-terminus of subunit II extends below the plane of the membrane and occupies the same region in space as the head groups of these lipids (Figure 3C). These examples, and the translation of OLC7 in cluster 5, represent ways in which lipid
Characterization of SLCO5A1/OATP5A1, a solute carrier transport protein with non-classical function.
... confirming the importance of OATPs in the transport of drugs . The OATP5 family consists of the sub-family OATP5A where OATP5A1 represents the only member in human, rat and mouse . The putative OATP5A1 polypeptide contains 848 amino Introduction The organic anion transporting polypeptide (OATP) family belongs to the gene superfamily of solute carriers ... 19 positive bile ducts . An expression profile of OATP5A1 protein can be viewed at ‘http://www.proteinatlas.org/tissue_profile. php?antibody_id = 25062’, with the strongest expression in cortical cells of the adrenal gland and follicle cells of the ovary, though the specificity of the used antibody needs to be determined. Although the expression profile ... represent the high-mannose and complex-glycosylated form of SLCO5A1, respectively. It has to be noted that the 89 kDa band was seen as a plasma membrane-bound protein in most of the experiments (Fig. 2B, upper panel) though not in the experiment displayed in Fig. 2A. The complex-glycosylated SLCO5A1 polypeptide is by far the most abundant SLCO5A1 species
Reduced BMP signaling results in hindlimb fusion with lethal pelvic/urogenital organ aplasia: a new mouse model of sirenomelia.
... the pelvic/urogenital organs of Bmp4 cKO mice. Bmp4 cKO mice had bladder aplasia, hypoplastic kidney, hypoplasia of external genitalia and anal stenosis (Fig. 2 B, D, F). These results suggest that disruption of Bmp4 function in caudal body regions phenocopies all prominent phenotypes of sirenomelia observed in humans. During mouse development, a transient embryonic ... Bmp4 cKO mice have both hindlimb fusion and defective pelvic/urogenital organs indicating that BMP4 is the critical ligand for caudal body development. Bmp7 KO mice do not show sirenomelia phenotypes (Fig. 6 B, F), but display kidney hypoplasia , ; implying possible functional redundancy of BMP signaling. Indeed, loss of a single copy of Bmp4 ... for pelvic/urogenital tissues. Normally, Bmp4 is expressed and BMP signaling is active in the aPCM during caudal body formation. Loss of pSMAD immunoreactivity in the aPCM region of Bmp4 cKO mice indicates that autocrine BMP4 action is required to form the aPCM. To investigate the role of BMP4 in the aPCM formation, we assessed apoptosis and cell proliferation
Selectivity Profiling and Biological Activity of Novel β-Carbolines as Potent and Selective DYRK1 Kinase Inhibitors.
... Reactions were stopped by pipetting aliquots of the reaction mix onto P8 1 phosphocellulose paper and immediate immersion in 5% phosphoric acid. Phosphocellulose papers were washed at least 5 times and radioactivity was measured by scintillation counting. Background values from samples incubated without kinase were subtracted and phosphate PLOS ONE | DOI:10.1371/journal.pone.0132453 ... phosphorylated upper band and the lower band. B, HEK293 cells with constitutive expression of GFP-tau and regulatable expression of GFP-DYRK1A were treated with doxycyclin and the indicated inhibitors for 18 h. Phosphorylation of tau on Thr212 was detected with a phosphospecific antibody. Expression levels of GFP-tau and GFP-DYRK1A were assessed with a GFP antibody. ... phosphorylated upper band to the lower band. Curve fitting for IC50 determination was done with the help of the GRAPHPAD PRISM 5.0 program (GraphPad Software, La Jolla, CA, USA). PLOS ONE | DOI:10.1371/journal.pone.0132453 July 20, 2015 14 / 18 Novel β-Carboline DYRK1A Inhibitors Supporting Information S1 File. Table A. Kinome profiling of AnnH75. Table
Description and implementation of a MiXed Layer model (MXL, v1.0) for the dynamics of the atmospheric boundary layer in the Modular Earth Submodel System (MESSy)
... wwilt 1 = exp (gD VPD) (48) f3 (VPD) 1 = 1.0 − 0.0016(298.0 − T )2 , (49) f4 (T ) 2.7.3 Evapotranspiration calculation The total evapotranspiration consists of three parts: soil evaporation, leaf transpiration and evaporation of liquid water from the leaf surface. The total evapotranspiration is therefore proportional to the vegetated fraction of the ... entrainment flux is the product of the entrainment velocity we (defined positive in the upward direction) and the potential temperature jump 1θ at the inversion. First-order closure approaches exist, which include the explicit representation of the depth of the entrainment zone, but the ZOJ approach already gives satisfactory results (Pino et al., 2006). The ... MXL/MESSy free troposphere entrainment gas/particle partitioning chemical conversion boundary height layer land surface sunrise deposition emission time sunset Figure 3. Processes relevant to evolution of species concentrations in the boundary layer. Open and closed circles depict gas-phase and aerosol-phase species, respectively. For the implementation of
Genetic characterization of complete open reading frame of glycoprotein C gene of bovine herpesvirus 1
... www.veterinaryworld.org/Vol.6/Nov-2013/13.pdf Figure-1. Result of colony PCR for confirmation of presence of amplified glycoprotein C gene insert using universal M13 primers. Lane 1: 100 bp plus DNA marker Lane 2: Colony PCR product Figure-2. Phylogenetic analysis of glycoprotein C gene of various isolates of BoHV1 with other related members of Alphaherpesvirinae showed ... characterization of complete ORF of glycoprotein C gene (UL44) of Indian isolate of BoHV1. Addition of glycerol is essential for amplifying GC rich portion of BoHV1 genome. Glycoprotein C gene of BoHV1 is highly conserved in all the isolates and it can be used as a target for designing of primers for diagnosis of IBR. Authors’ contributions SM, ABP and MAR implemented ... PCR mixtures to enhance the speci? city and/or the yield of the PCR from high GC templates . The G+C content of BoHV1 ranges from 71-72% . Glycerol helps in strand-separation and primer-annealing temperatures and also reduces secondary structure that could inhibit the progress of the polymerase. It lowers the temperature of strand separation by lowering
Subjective Preferences of Criterion-Oriented Support of Professional Activities of Managers
... underwent a preliminary quantitative and qualitative analysis. A complex interdisciplinary approach was used in this research. Keywords: professional experience, a criterion-oriented support of professional activities of managers; assessment of professional activities effectiveness. While accumulating professional experience, employees develop and improve ... managers; standards and regulati new ideas and offers; ons, plan completion development of existing directions; progress control; performance of job duties; appraisal of an inner customer; program execution prevention of violations correction of errors; absence of standstills; provision of of standards, prevention continuous work of deviations and losses 366 Fairuza ... (degree) of managerial ability to generate profit and operate costs”. In this case, a productivity formula can be represented by the following ratio: profit before fixed charges x 100 ÷ Sales revenue. The problem of such approach to assessment of operating efficiency of a manager in Russian companies can be explained by two reasons. Firstly, such approach
Essential oil content and composition of aniseed
... Pimpinella anisum L. Eur. J. Exp. Biol. 2: 1326–1336. Naher S, Ghosh A, Aziz S (2012): Comparative studies on physicochemical properties and GC-MS analysis of essential oil of the two varieties of the aniseed (Pimpinella anisum Linn.) in Bangladesh. Int. J. Pharm. Phytopharmacol. Res. 2: 92–95. 73 Orav A, Raal A, Arak E (2008): Essential oil composition ... Simon JE, Widrlechner MP (1995): Characterization of essential oil of dill (Anethum graveolens L.). J. Essent. Oil Res. 7: 11–20. Darzi MT, Haj S, Hadi MR, Rejali F (2012): Effects of the application of vermicompost and phosphate solubilizing bacterium on the morphological traits and seed yield of anise (Pimpinella anisum L.). J. Med. Plants Res. 6: 215–219. Gil ... Effects of phosphate solubilizing microorganisms and plant density on seed yield and essential oil content of anise (Pimpinella anisum). Middle-East J. Sci. Res. 14: 940–946. Zehtab-Salmasi S, Javanshir A, Omidbaigi R, Alyari H, Ghassemi-Golezani K (2001): Effects of water supply and sowing date on performance and essential oil production of anise (Pimpinella
A biophysical model of endocannabinoid-mediated short term depression in hippocampal inhibition.
... Biophysical Model of Endocannabinoid-Mediated Short Term Depression in Hippocampal Inhibition Margarita Zachariou1*, Stephen P. H. Alexander2, Stephen Coombes3, Chris Christodoulou1 1 Department of Computer Science, University of Cyprus, Nicosia, Cyprus, 2 School of Biomedical Sciences, University of Nottingham, Nottingham, United Kingdom, 3 School of ... and memory. Here, we present a biophysically plausible model of cannabinoid retrograde signalling at the synaptic level and investigate how this signalling mediates depolarisation induced suppression of inhibition (DSI), a prominent form of shortterm synaptic depression in inhibitory transmission in hippocampus. The model successfully captures many of the key characteristics ... reduction of GABA release by retrograde signalling from a strongly depolarised post-synaptic cell to the pre-synaptic GABA-releasing cell [12,13]. DSI, a widely observed CB-mediated phenomenon, was first reported in hippocampus  as well as cerebellum . eCBs can also mediate depolarisation-induced suppression of excitation (DSE) in the hippocampus
Parallel Implementation of Bias Field Correction Fuzzy C-Means Algorithm for Image Segmentation
... defined per percentage and given as speedup ratios performance (SRP): So, for each GPU device we have: Fig. 9. Speedups of the parallel implementations over the serial one for Lena image size of. 6.5536 million pixels C. Application example To illustrate the effectiveness of the proposed PBCFCM algorithm we present in fig.10 an application example on ... community by using the power of parallel calculations that are well suited to this type of card. Graphic processing units (GPUs) were originally created for rendering graphics. Recently, GPUs has emerged as coprocessing units for Central Processing Units (CPU) and has become popular for general-purpose high performance computation (GP-GPU) which is mainly attractive ... parallel implementation of the algorithm and different GPU. Performance improvement is manifested on the three curves of the speed-up to an x64 configuration. The maximum values of speed-up obtained are of the order of 63x, 41x and 19x for GTX 580, GTX 760 and GT 740 respectively for image size up to about to 7 Mega pixels. Fig. 5. Speed-up comparison
Detection/Removal of Cooperative Black and Gray Hole Attack in Mobile Ad-Hoc Networks
... USA overloaded, lack of CPU cycles, buffer space or bandwidth to forward packets. For these the above straight forward comparison cannot be applied in a rigorous manner. Therefore we assume a threshold probability of packets dropped by a node through no fault of its own. Let μ be the threshold probability of non malicious packet drop by each node then ... itself droping all the packects Step 220.127.116.11: Reply “NRREP Malicious” to S. Step 2.2.2: Else Step 18.104.22.168: Reply result (MN, S, NRREP) to S, which means NRREP may be malicious. Step 22.214.171.124: Broadcast query(S, D, NRREP, ni) message to all its neighbors replacing NRREP by Nnext and ni by dataCount for NRREP. Step 3: If the receiving node is not neighbor of ... Remove NRREP from FindMalicious table and append NRREP in Gray/BlackHole table. Step 126.96.36.199: Broadcast “NRREP Malicious” to the Network. Step 188.8.131.52: Set findHoleStatus = true in the routing table of S for the route to D. Step 3.2: Else Step3.2.1: Append NRREP in FindMalicious. Step 3.2.2: Initialize voteCount = 1. Step 4: Initialize j = 1. Step 5: When
Evaluation of the JULES land surface model in simulating catchment hydrology in Southern Africa
... multiple acceptable outcomes is discussed in detail by Beven and Freer (2001) and Beven (2006). An alternative approach for determining model parameters is to employ a reduced parameter model where parameters are estimated a priori from relationships to measured physical properties such as soil characteristics or topography. It is the latter approach that ... a shape parameter which determines the relative proportion of shallower to deeper stores. A higher value of b results in fewer high capacity (“deep”) stores relative to low capacity (“shallow”) stores and so will result in a more rapid production of surface runoff. In TOPMODEL (Beven and Kirkby, 1979; Beven et al., 1995; Beven, 1997), a topographical ... MacKellar et al. Title Page Abstract Introduction Conclusions References Tables Figures J I J I Back Close | 20 Discussion Paper 15 | 10 Discussion Paper 5 The major shortcoming of TOPMODEL in the upper Orange catchment is its gross underestimation of river flow magnitude. A possible cause of this is inappropriate topographical index ancillary
Expression of fibroblast growth factor-21 in muscle is associated with lipodystrophy, insulin resistance and lipid disturbances in patients with HIV.
... in Copenhagen. LD was defined clinically by physical examination of peripheral lipoatrophy (defined by the presence of peripheral lipoatrophy with at least one moderate sign of fat loss in face, arms, buttocks, or legs based on a physical examination by a single investigator (BL) using a validated questionnaire developed by Carr et al. . Some of the ... Biosystems). Sequence-specific FGF-21 primer/probe sets were used for FGF-21 amplification, while predeveloped household TaqMan primer/probe sets (Applied Biosystems) were used for 18S detection. The relative expression of FGF-21 was normalized to the endogenous control and expressed as FGF-21 expression per 18S expression. The levels of 18S in the skeletal ... resistance, Rate of appearance and disappearance, Rate of appearance and disappearance of glucose during a euglycemic-hyperinsulinemic clamp performed in both HIV patients and healthy controls. *P, 0.05; * *P, 0.01; ** *P, 0.001, *** *P, 0.0001 by t-test. doi:10.1371/journal.pone.0055632.t001 PLOS ONE | www.plosone.org 4 March 2013 | Volume 8 | Issue 3 |
The Divergent Intracellular Lifestyle of Francisella tularensis in Evolutionarily Distinct Host Cells.
... species and antimicrobial peptides . Interestingly, inhibition of the acidification by a specific inhibitor of the vATPase proton pump, bafilomycin A, does not block, but delays, bacterial escape to the cytosol (Fig 1A) . It appears that acidification of the FCP enables rapid phagosomal membrane disruption and bacterial egress in the macrophage ... mannose-6-phosphate, and lysosomal associated proteins (LAMPs), but the FCP does not mature into a phagolysosome [17,24,25]. The FCP is acidified through acquisition of the vATPase proton pump [23,25]. This is followed by escape of the bacteria to the cytosol within 30 to 60 minutes of entry [17,25]. The short time spent in the FCP is a dynamic step in the ... a type VI Secretion System (T6SS), since the FPI-encoded proteins IglA, IglB, VgrG, PdpB, and DotU show similarity to structural components of T6SS of other bacteria [10,11]. The existence of the FPI-encoded secretion system is supported by finding that IglI and VgrG proteins are secreted by F. novicida to the cytosol of macrophages . A lipoprotein
Reduction of canine plasminogen leads to an expanded molecule which precipitates.
... influence of TCEP on the circular dichroic spectrum of DPGN. The top panel shows that the far UV spectra down to 190 nm is not influenced by TCEP at ratios of TCEP to DPGN of 0, 2, 4 or 8. The protein concentration was 3.2 mM in the 0 TCEP sample and 2 mM in the 16 mM TCEP sample. At these concentrations, the plasminogen did not precipitate. The bottom panel ... during synthesis of protein by HEPG2 cells [28,29]; when synthesis occurs in the presence of dithiothreitol, there is formation of aggregates which contain several different protein species .This study shows that one or two of the 24 disulfide bridges in plasminogen are especially labile and, on reduction, expose groups which bring about precipitation of ... Forloni G (2005) Role of plasminogen in propagation of scrapie. J Virol 79: 11225–11230. 16. Wiman B, Wallen P (1975) Proceedings: On the primary structure of human plasminogen and plasmin, cyanogen bromide fragments. Thromb Diath Haemorrh 34: 350. 17. Wiman B, Wallen P (1975) On the primary structure of human plasminogen and plasmin. Purification and
Government borrowing and the long-term interest rate: Application of an extended loanable funds model to the Slovak Republic
... incorporated in this study increase the explanatory power of the behaviour of the government bond yield. There are several policy implications. The significant coefficient of the ratio of government borrowing to GDP implies that pursing expansionary fiscal policy to stimulate the economy would raise the long-term government bond yield and crowd out part ... Economic Journal, Volume 56, pp. 802-803. Raynold, P. (1994), ‘The impact of government deficits when credit markets are imperfect: evidence from the interwar period’, Journal of Macroeconomics, Volume 16, pp. 55-76. Romer, D. (2000), ‘Keynesian macroeconomics without the LM curve’, Journal of Economic Perspectives, Volume 14, pp. 149-160. Saleh, A. S. ... funds model. Hence, the inclusion of the EU government bond yield and the nominal effective exchange rate increases the value of adjusted R-squared of the regression and improves the explanatory power of the behaviour of the Slovak government bond yield. 4. SUMMARY AND CONCLUSIONS This paper has applied an extended open-economy loanable funds model
A new class of pluripotent stem cell cytotoxic small molecules.
... apoptosis. Signalling intermediates downstream of all 3 main UPR receptor pathways have been identified as having pro-apoptotic roles [22,23]. The 3 principal UPR receptors involved in the UPR apoptosis cascade are Ire1, ATF6 and PERK. Microarray analysis further revealed that the downstream components and genes of the PERK/ATF4/ DDIT3 pathway in particular ... be present in the top 50 upregulated list of genes (Fig. 5). The ER stress response, also known as the unfolded protein response (UPR), is a cellular stress mechanism activated in response to an accumulation of mis-folded proteins in the lumen of the endoplasmic reticulum [22,23]. In conditions of prolonged stress, UPR commits the cell to a pathway of ... JC011 (A). Top 10 components of the PERK/ ATF4/DDIT3 ER stress pathway that were found to be rapidly upregulated in JC011 treated BGO1V cells (B). qRT-PCR confirmation of upregulated UPR/ER stress pathway genes following JC011 treatment (C). doi:10.1371/journal.pone.0085039.g005 PSC contamination in differentiated cell populations by up to 6fold (Fig.
Seasonal distribution of dissolved inorganic carbon and net community production on the Bering Sea shelf
... from primary production is highest over the Middle and Coastal Domains (Grebmeier and McRoy, 1989; Haﬂinger, 1981), where smaller species of copepods typical of the region inshore of the central front are incapable of completely grazing the prodigious volume of 20 production in the “Green Belt” (Cooney, 1981) and the rapid volume of production of the ... rates of PP for the Bering Sea shelf Because of the importance of Bering Sea shelf ﬁsheries, there have been numerous studies of pelagic primary production over the southeast shelf (Table 1). The ﬁrst eﬀorts in this region began in the early 1960s, using the abundance of ﬁsh supported by the 10 ecosystem to infer the necessary amount of primary production ... Materials (CRMs, provided by A.G. Dickson, Scripps Institution of Oceanography) and repeat sampling ensured that the accuracy of the DIC measurements was within 0.05% and was stable over time. 3.3 Estimates of NCP 10 In our approach, we exploit seasonal changes in biological reactants and products (e.g., DIC), to estimate rates of NCP and identify
Introduction: Observations and Modeling of the Green Ocean Amazon (GoAmazon2014/5)
... n/a Description Forested site upwind of Manaus, 325 m tall tower (inaugurated Aug 2015) Pasture site north of Manaus Closed canopy, forested site north of Manaus Broken canopy, forested site north of Manaus On INPA campus in Manaus Ponta Pelada Airport (PLL) Adjacent to river, just downwind of Manaus; river width of 7.5 to 11.7 km depending on direction of prevailing ... Source: http://www.arl.noaa.gov/HYSPLIT_info.php; last access: 15 August 2015. prevailed in the atmospheric boundary layer of the Manaus region (Fig. 4a1 and a2). Regular and strong precipitation events are apparent in the time series of daily rainfall amounts (Fig. 4a3). Deep tropospheric moistening because of regular large rain events is apparent (Fig. ... introduction presented herein, research results and ﬁndings of GoAmazon2014/5 appear in the articles of this special issue. The special issue is open for new submissions for 3 years. Overview articles are planned for summarizing and synthesizing the ﬁndings of the papers of the special issue. These planned overviews encompass the several objectives of GoAmazon2014/5. The
SIMULATION OF CNTFET DIGITAL CIRCUITS: A VERILOG-A IMPLEMENTATION
... ECp, for ECp 0.5 eV, have the following empirical expressions : Ap 5.3E 2 Cp 10ECp 1 Bp 0.34ECp 1 Therefore the quantum capacitances CGD and CGS are given by: CGD q ? ?p n Dp q VGS p n Dp Dp VCNT Dp VCNT VGS CGS q ? ?p nSp q VGS p nSp Sp VCNT ... the number of carrier nip (i = S or D), which increases almost linearly as ip greater or equal than zero and falls off exponentially as ip becomes negative, can be derived from the following relationship : Apexpip nip N 0 Bpip Ap for ip 0 for ip 0 i S, D (8) where the parameters Ap and Bp, depending ... IDSp 4qkT h ln 1 exp Sp ln 1 exp Dp (1) where k is the Boltzmann constant, T is the absolute temperature, h is the Planck constant, p is the number of sub- bands, while Sp and Dp have the following expressions: Sp qVCNT kT ECp and Dp qVCNT ECp kT qVDS (2) being ECp the