Proteasome Inhibition Contributed to the Cytotoxicity of Arenobufagin after Its Binding with Na, K-ATPase in Human Cervical Carcinoma HeLa Cells.

... bufadienolide isolated from toad venom, on human cervical carcinoma HeLa cells was checked. And, the protein expression profile of control HeLa cells and HeLa cells treated with arenobufagin for 48 h was analyzed using two-dimensional electrophoresis, respectively. Differently expressed proteins in HeLa cells treated with arenobufagin were identified and the ... Cytotoxicity of arenobufagin (Arg) on HeLa cells. (A) Chemical structure of arenobufagin. (B) Cell viability of HeLa cells treated with arenobufagin at different doses for 24 h, 48 h and 72 h. (C) Representative DNA histograms of HeLa cells obtained by flow cytometry analysis. Accumulation in G2/M phase was observed in HeLa cells treated with 100 nM arenobufagin ... shown in Fig 1, arenobufagin exhibited the cytotoxicity against HeLa cells. Arenobufagin dose-dependently and time-dependently decreased the viability of HeLa cells by MTT assay (Fig 1B). Arenobufagin caused G2/M phase arrest and apoptosis of HeLa cells. The representative DNA histograms of HeLa cells exposed to arenobufagin by flow cytometry assay showed
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tRNA modifying enzymes, NSUN2 and METTL1, determine sensitivity to 5-fluorouracil in HeLa cells.

... rapid tRNA(ValAAC) degradation. A similar situation may therefore occur when exposing HeLa cells depleted of NSUN2 and METTL1 to 5FU. To address this question we performed tRNA stability assays using HeLa cells with double knockdown of NSUN2 and METTL1 and as control HeLa cells transfected wit scramble shRNA. As shown in Figure 5, tRNA(ValAAC) was unstable ... Sensitivity in clone #3-3. (A) In vitro growth curves of these clones (#1-7, #2-1, #2-8 #3-3, and #3-5) and parental HeLa cells. (B) Colonyforming abilities of these clones (lanes #1-7, #2-1, #2-8 #3-3, and #3-5) and parental HeLa cells (lane HeLa) in 0.2% washed agar medium. All experiments to obtain quantitative data were repeated independently three times ... cells [27]. However, in our previous studies, NSUN2 expression was not altered during the cell cycle of HeLa cervix carcinoma cells [17]. When we investigated normal human diploid fibroblasts, NSUN2 expression was found to be very low compared with HeLa cells and again NSUN2 was not differentially expresses during the cell cycle [17]. In initial studies
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Evaluation of the effects of paederus beetle extract and gamma irradiation on HeLa cells

... treated with PBE alone. Colony – forming assay Survival fractions of HeLa cells were decreased by increasing doses of gamma radiation and gamma 304 Iran J Basic Med Sci, Vol. 17, No. 4, Apr 2014 Samani et al Effects of paederus beetle on Hela cells Figure 1. Survival curve of HeLa cells exposed to gamma radiation radiation at doses higher ... radiation on cancerous cell lines. Therefore, this study was designed to evaluate the potential effects of the combination of PBE and ionizing radiation on HeLa cells. Materials and Methods Cells and reagents HeLa cell line was obtained from Iran Pasteur Institute, Tehran, Iran). The RPMI 1640 medium, fetal bovine serum (FBS) and PenStrep® (penicillin10000 ... fraction compared to control group as shown in Figure 1. Survival fractions of HeLa cells were decreased by increasing concentrations of PBE and except for the lowest concentration of PBE, the viability was significantly lower than the control group, (Figure 2). Survival curve of HeLa cells treated with the combination of different concentrations of PBE
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Aderência e invasibilidade em células HeLa de amostras de Campylobacter spp. isoladas de animais

... com gentamicina, em células HeLa. Sessenta e três amostras foram testadas quanto à sua capacidade de aderência e invasão, pelo ensaio com gentamicina, em células HeLa. Os resultados demonstraram que amostras de C. jejuni, C. coli e C. fetus subsp. fetus, isoladas de fezes de animais são capazes de aderir e invadir as células HeLa, porém nenhuma amostra ... 13 amostras (41%) aderiram e invadiram (Ad+ / Inv+) as células HeLa. Quase a metade das amostras testadas (49%) não foi capaz nem de aderir, nem de invadir as células HeLa (Tab. 2). 15 Tabela 2- Aderência e invasão de amostras de Campylobacter sp. isoladas de animais em células HeLa. ESPÉCIE C. jejuni Sub-total ORIGEM frango cão suíno bezerro ... invasivas (Ad+ / Inv+) em células HeLa segundo a origem da amostra. Dentre as 19 amostras de C. jejuni testadas, duas (10,5%) foram capazes de aderir (Ad+/ Inv-) e três (15,7%) invadiram (Ad+ / Inv+) as células HeLa. Dentre as 15 amostras de C. coli, três aderiram (Ad+/ Inv-) e outras três (20,0%) invadiram (Ad+ / Inv+) as células HeLa. Das 29 amostras de C.
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O uso da cultura celular (Hela) para triagem de novas drogas com ação "antitumoral".

... òincz 19'60, by Ά (National CanceA Institute) hoi, been òlightly moat^ied. It haí> been uu>e.d Hela ti culture {cell tine derived {΅rom human carcinoma o¿ uteruÁ) instead o{¡ the original [cell tine. derived {΅rom human carcinoma o{ nasopharynx) . Hela cellò weAe cultivated Eagle1 ò ba&al medium pluÁ 10% òerum added o{΅ anttbiotick: penicillin 100 ... a l . , 1972) e nas suas a t u a l i z a ç õ e s ( I n s t r u c t i o n 14, 1980),uti 1 izamos a mesma metodologia introduzindo como modificação o emprego da linhagem celular Hela. 1 A c u l t u r a de c é l u l a s e s t a c i o n a r i a s é c u l t i v a d a e m m e i o basal d e Eag 1 e e ad icionada d e 10¾ de soro de v i t e l o , c o n t e n ... O USO D A C U L T U R A C E L U L A R (Hela) P A R A T R I A G E M D E N O V A S D R O G A S C O M A Ç Ã O " A N T I T U M O R A L " . B e a t r i z D. O. M i r a n d a d a C r u z (*) M a r i l d a M e i r e l l e
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