Biochemical characterization of CTX-M-15 from Enterobacter cloacae and designing a novel non-β-lactam-β-lactamase inhibitor.
... analysed from Lineweaver-Burg plot. inactive degradation product of the substrate. The parameters k+2, k+3 and K9 are the first-order acylation and deacylation constants, and the dissociation constant of the Henri-Michaelis complex, respectively. The steady-state kinetic parameters for the above equation are given by the following equations: Stability ... inhibitors. Steady-state Kinetic Parameters Determination The steady-state kinetics of the purified CTX-M-15 was carried out on nitrocefin, ampicillin, piperacillin, cefazolin, cefuroxime, cefotaxime, ceftriaxone, ceftazidime, cefepime and aztreonam. The representative Michaelis-Menten plots are shown in figure S2, and the deduced kinetic parameters (kcat, Km and ... from MICs of blaCTX-M-15 expressing E. coli DH5a transformed cells. The enzyme kinetics parameters of CTX-M-15 in the presence of various inhibitors are presented in table 4. CTX-M-15 was efficiently acylated by clavulanic acid, sulbactam and tazobactam, and individual kinetic parameters were computed. The catalytic activity of CTX-M-15 on clavulanic acid,
Comparison of microbiological, histological, and immunomodulatory parameters in response to treatment with either combination therapy with prednisone and metronidazole or probiotic VSL#3 strains in dogs with idiopathic inflammatory bowel disease.
Current state-of-the-art for the measurement of non-Maxwellian plasma parameters with the EISCAT UHF Facility
... conditions it is necessary to simultaneously fit seven independent parameters to analyse one ionic radar spectrum: these are electron density, electron temperature, ion composition and two effective non-Maxwellian shape distortion parameters for both O` and NO` ion species. Our attempts to fit so many parameters led to ambiguous results. Then, we proposed a scenario ... constant, * m the ion mass and ¹*u , D* u are shape parameters * (Kikuchi et al., 1989). From this model of distribution we obtain the line-of-sight temperature as given by ¹u¹* u (1#D*2 u ). (2) This fitting process contains four parameters: the electron density, the electron temperature and two effective parameters ¹* u , D* u . Table 1 presents, for ... become saturate of for large electric field inten90 sities, like the other shape parameters D* u for 30°4u( Fig. 3. Shape parameters D* u of the NO` line-of-sight velocity distribution as a function of the electric field intensity. All curves saturate for all angles u 90°. The parameters D*u also increase with an increasing u whatever the electric field
Dance type and flight parameters are associated with different mushroom body neural activities in worker honeybee brains.
... Dance Type and Flight Parameters Are Associated with Different Mushroom Body Neural Activities in Worker Honeybee Brains Taketoshi Kiya*¤, Takeo Kubo Graduate School of Science, Department of Biological ... suggest that the foraging frequency and visual experience during foraging are associated with different MB neural activities. Citation: Kiya T, Kubo T (2011) Dance Type and Flight Parameters Are Associated with Different Mushroom Body Neural Activities in Worker Honeybee Brains. PLoS ONE 6(4): e19301. doi:10.1371/journal.pone.0019301 Editor: Daniel Osorio, ... were observed in the field experiments. The discrepancy between these experiments implies that kakusei-signal density in the MBs is not a simple reflection of the foraging flight parameters, but rather varies depending on the experimental conditions. This variability might be one of the reasons that we could not detect a significant relationship between
Quality assessment of water cycle parameters in REMO by radar-lidar synergy
... large as 200 m (Hennemuth and Lammert, 2006). Cloud parameters Scattering of millimeter waves is particularly suited to characterise cloud parameters because most clouds can on one hand be detected and on the other hand are penetrated, even if there are multiple optically thick cloud layers. Cloud parameters to be determined by radar are cloud cover, ... of8000 water cycle parameters in REMO by radar-lidar synergy km-1 B. Hennemuth et al.: Quality assessment of water cycle parameters in REMO by Radar-Lidar synergy 21.3 Height, m 6 6000 20.4 2000 23.117.7 21.3 multiple cloud layers areassessment pene24.0 6 Figure 3 illustrates that B. Hennemuth et al.: Quality of12000 water cycle parameters in REMO ... evaluation of the water cycle simulated by a model with observations is tried. The water cycle parameters measured at the Meteorological Observatory Lindenberg (MOL), Germany, during experiments in summer and autumn 2003 and 2004 are compared with simulated parameters of the regional model REMO. The comparisons include vertical distribution of humidity
ariação e parâmetros genéticos em dois bancos de germoplasma de Tabebuia heptaphilla (Velloso) Toledo. Variation and genetic parameters in two germplasm banks of Tabebuia heptaphilla (Velloso) Toledo.
... baixa variação genética e pequeno número status retido em ambos os bancos de germoplasma. The aim of this study was to investigate the genetic variation and to estimate genetic parameters for growth, form and survival traits at 22 and 21 years of age in two progeny tests of Tabebuia heptaphylla, established in Luiz Antônio Experimental Station, belonging
Biochemical and physiological effects of phenols on human health
... Biochemiczne i fizjologiczne skutki oddzia∏ywania zwiàzków fenolowych na organizm cz∏owieka Biochemical and physiological effects of phenols on human health Danuta Wojcieszyƒska, Urszula Guzik, Katarzyna Hupert-Kocurek, Julita Marsza∏ek Uniwersytet Âlàski, Katedra Biochemii. Kierownik
Evaluation of Machining Parameters Influencing Thrust Force in Drilling of Al– SiC–Gr Metal Matrix Composites using RSM
... are three machining parameters i.e. Spindle speed, Feed rate, Drill diameter. Experiments are conducted on a vertical machining centre using Taguchi design of experiments. Taguchi orthogonal array is designed with three levels of drilling parameters with the help of software Minitab 15. A model is developed to correlate the drilling parameters with thrust ... drilling parameters on Thrust force in drilling Al/SiC/Graphite hybrid metal matrix composites. The experiments were conducted on a CNC Vertical machining centre using Multifaceted drills of diameter 4mm,8mm and 12mm. Response surface model is developed to correlate the thrust force with respect to different drilling parameters. The machining parameters considered ... mechanical structures. Many researchers [8-10] reported that the quality of the drilled surfaces depend strongly on the tool geometry, drilling parameters and tool material. An inappropriate selection of these parameters can lead to material degradations, such as fiber pull-out, matrix cratering, thermal damage and delamination . Latha and Senthilkumar
BIOCHEMICAL AND PHYLOGENETIC STUDIES OF CreD OF Corynebacterium glutamicum
... BIOCHEMICAL AND PHYLOGENETIC STUDIES OF CreD OF Corynebacterium glutamicum Muhammad Tausif Chaudhry*1, Raheela Chaudhry1, Shuang-Jiang Liu2 Address(es): 1 National Physical and ... with conserved HD domain. In the present report metal dependence before and after chelation, and thiol inhibition on phosphohydrolase activity of CreD were studied. In addition, kinetic parameters were determined and analysis of sequence data and phylogeny was performed. MATERIAL AND METHODS 200 GL column (GE Healthcare, UK) eluted with 50 mM Tris-HCl (pH 7.6) containing ... before addition of 4-NPP (4 mM) and further incubated for 10–20 min before recording A410. Both positive and negative controls were included in each set of experiments. Kinetic parameters Protein expression, purification and molecular mass determination CreD was expressed and initially purified as described (Li et al., 2014) and then purified by SuperdexTM
The experimental effect of artificial air ionizer (negative and positive) on some hematological parameters at Wistar rats
... Balneo-Research Journal Vol.2, Nr.1, 2011 THE ARTIFICIAL AIR IONIZATION EFFECT (NEGATIVE AND POSITIVE) IN EXPERIMENT ON SOME HEMATOLOGICAL PARAMETERS OF WISTAR RATS * Iu. Simionca, L.Enache National Institute of Rehabilitation, Physical Medicine and Balneoclimatology 1. INTRODUCTION 1.1. General considerations ... the body. These influences are possible because living organisms manifests itself in a very large number of phenomena such as electricity with essential biological role (e.g, biochemical reactions, transmembrane transport of the substance of the nervous impulse propagation and others). However, based on these findings, it was concluded that under the ... components (RBC, some colloids), directly altering their electrical properties and stability and indirectly affecting other properties of various organic structures through numerous biochemical reactions that contribute to increased metabolism. The studies and the research conducted till now indicates many biological and therapeutic effects. Among those
Biochemical Characterization of a Family 15 Carbohydrate Esterase from a Bacterial Marine Arctic Metagenome.
... a11111 RESEARCH ARTICLE Biochemical Characterization of a Family 15 Carbohydrate Esterase from a Bacterial Marine Arctic Metagenome Concetta De Santi, Nils Peder Willassen, Adele Williamson* The Norwegian ... of Chemistry, UiT—The Arctic University of Norway, Tromsø, Norway * firstname.lastname@example.org Abstract OPEN ACCESS Citation: De Santi C, Willassen NP, Williamson A (2016) Biochemical Characterization of a Family 15 Carbohydrate Esterase from a Bacterial Marine Arctic Metagenome. PLoS ONE 11(7): e0159345. doi:10.1371/journal.pone.0159345 Editor: ... enzymes are predicted in the genomes of several bacteria, however these have been much less studied than their fungal counterparts. Here we describe the recombinant production and biochemical characterization of a bacterial CE15 enzyme denoted MZ0003, which was identified by in silico screening of a prokaryotic metagenome library derived from marine Arctic
Constraining model parameters on remotely sensed evaporation: justification for distribution in ungauged basins?
... parsimony in model parameters is often advocated to prevent the undesirable occurrence of equiﬁnality (e.g. Beven and Binley, 1992; Beven and Freer, 2001; Savenije, 2001). Although parsimony results in simple and to a certain extent identiﬁable models, their predictive capacity, for instance of land cover changes, is rather small, because parameters usually ... all parameters, their physical meaning and unit is given in Table 1. Note that in this study, the focus is only on the soil reservoir of the model, not on the runoff generating reservoirs. An upward ﬂux from the runoff generating reservoirs to the soil reservoir was deliberately excluded. This results in a model structure in which there are only 2 parameters, ... and 4.5, linearly depending on the normalised soil texture between 0 and 1. Also the routing parameters Fperc, Ks and Kq do not inﬂuence our results, because there is no feedback from the discharge generating reservoir towards the soil moisture. Therefore these parameters are not mentioned in the remainder of this article. Daily rainfall input was taken
Biochemical composition and germination capacity of Ligustrum lucidum ait. seeds in the process of biological invasion=Composição bioquímica e capacidade de germinação de sementes de Ligustrum lucidum ait. no processo de invasão biológica
... Acta Scientiarum http://www.uem.br/acta ISSN printed: 1679-9283 ISSN on-line: 1807-863X Doi: 10.4025/actascibiolsci.v34i3.10641 Biochemical composition and germination capacity of Ligustrum lucidum ait. seeds in the process of biological invasion Aquélis Armiliato Emer1, Marisa de Cacia Oliveira1*and
Biochemical and ultrastructural changes in the liver of European perch (Perca fluviatilis L.) in response to cyanobacterial bloom in the Gruža reservoir
... and fish. In the present study P. fluviatilis was chosen as the test organism to investigate the influences of the A. flos-aquae bloom in the Gruža Reservoir on biochemical and ultrastructural parameters in liver. We determined the specific activity of antioxidant defense enzymes: total, manganese and copper zinc containing superoxide dismutase (Tot SOD, ... the eutrophic status of the reservoir (Ranković and Simić, 2005). In this study, measurements of environmental parameters as well as determination and quantification of the phytoplankton community were performed at the time of fish sampling. Biochemical response of European perch to cyanobacterial bloom 981 The freshwater fish European perch ... and immediately killed by a blow to the head. The liver was rapidly dissected out and frozen at -80°C for biochemical analysis. For histopathological study, the liver was immediately fixed in 10% neutral-buffered formalin until further processing. Biochemical analysis The liver was minced and homogenized in 5 vol. (Lionetto et al., 2003) of 25 mmol/L
A New Method to Calculate Centrifugal Pump Performance Parameters for Industrial Oils
... is represented for calculating centrifugal pump performance parameters when the pump works with viscous fluids. In this way, mechanical, volumetric and disk friction losses are considered in calculating hydraulic efficiency. A computer program was developed for computing the pump performance parameters such as head, efficiency and input power for viscous ... computer program. The interesting point known previously as "sudden rising head" can be seen on such diagram. 2. CALCULATION OF PUMP PERFORMANCE PARAMETERS FOR VISCOUS FLUID PUMPING In this section, performance parameters of centrifugal pump such as head (H), efficiency (ߟ) and input power ( P ), for viscous fluid pumping in will be derived using new ... losses in all components of pump are made up by friction and turbulent dissipation losses. Friction losses are depended on working fluid viscosity and therefore pump performance parameters are highly affected by this parameter. It should be noted that this is because the flow regime of highly viscous fluids tends to be laminar, roughness of impeller side
Change of sensory characteristics and some quality parameters of mixed milk and cocoa spreads during storage up to 180 days
... data obtained in the investigations performed in this study were analyzed by descriptive and analytical statistics. Basic parameters of the descriptive statistics included calculations of the arithmetic mean values, and variabiltiy parameters of the investigated properties included determinations of standard deviations (Sd) and variation coefficients ... oiliness. 785 J.V. POPOV-RALJIĆ et al.: QUALITY PARAMETERS OF MIXED MILK AND COCOA SPREADS Hem. ind. 67 (5) 781–793 (2013) Figure 4. Box-plots for sensory property – flavor-odor. Figure 5. Box-plots for sensory property – flavor-taste. Figure 6. Box-plots for weighted mean value. 786 J.V. POPOV-RALJIĆ et al.: QUALITY PARAMETERS OF MIXED MILK AND COCOA ... production. Besides achieving the appropriate sensory properties (appearance, colour, surface, shine, consistence and flavor), as very important parameters for the con- 789 J.V. POPOV-RALJIĆ et al.: QUALITY PARAMETERS OF MIXED MILK AND COCOA SPREADS Hem. ind. 67 (5) 781–793 (2013) sumers, a considerable task for the confectionery industry is
NUMEREICAL ANALYSIS OF FOUR WAVE MIXING AND EXTRACTION OF DISPERSION PARAMETERS OF THE FIBRE
... separation between signal and pump source, which will be used to propose a power independent method for extraction of dispersion parameters of a fibre. Keywords: Four wave mixing (FWM), Efficiency, Power of Idler, and Dispersion Parameters I. INTRODUCTION The field of nonlinear optics was ushered in with the development of the first laser by Maimenin ... extract the dispersion parameters. Figure 4 shows the experimental setup to obtain the power of the idler. Fig. 4Experimental arrangement for finding out the power of idler as a function of wavelength separation Once the idler power graph is obtained for the fibre the following algorithm can be used to find the dispersion parameters. A. Algorithm ... description of all four-wave mixing processes. The experiment is performed using a highly non-linear fibre. The experiment classifies itself as a technique to calculate the dispersion parameters. The first input field causes an oscillating polarization in the dielectric which re-radiates with some phase shift determined by the damping of the individual dipoles;
Research on the changes of some physiological parameters in several fish species under the action of the talstar insecticide
... Drăghici, O., (2009): The Changes of Some Physiological Parameters in Prussian Carp Under The Action of the Tilt Fungicide. Bulletin UASVM, Cluj, 66(1-2): 47-52.  Ponepal, M.C., Păunescu, A., Marinescu Al. G., Drăghici, O., (2009): Effect of the Fungicide Chlorothalonil (Bravo) on Some Physiological Parameters in Prussian Carp. Lucrări ştiinţifice USAMV ... (Oncorhyinchus mykiss). Veterinarni Medicina, 51: 469-476.  Velisek, G., Svobodova, Z., Machova, J., (2009): Effects of bifenthrin on some haematological, biochemical and histopathological parameters of common carp (Cyprinus carpio L.). Fish Physiology and Biochemistry, 35: 583590.  Walden, C.C., Howard, T.E., Froud, G.C., (1970): A quantitative ... the toxicity of pyrethroids is little affected by pH or water hardness . The aim of this study is to analyze the influence of Talstar insecticide upon some physiological parameters (oxygen consumption, breathing frequency, number of red blood cells) in freshwater fishes: prussian carp (Carassius auratus gibelio Bloch), bleak (Alburnus alburnus
Haemato-biochemical profiles of indigenous goats (Capra hircus) at Chittagong, Bangladesh
... for haemato -biochemical parameters according to different species and age categories. The student t-test was used to compare the results of different haemato -biochemical parameters between goat types. The results were presented as mean with the standard deviation and p-value
Small changes in environmental parameters lead to alterations in antibiotic resistance, cell morphology and membrane fatty acid composition in Staphylococcus lugdunensis.
... the three environmental parameters in wound sites as summarized in Figure 1. The reference control samples were incubated at 37uC and pH 7, with no added NaCl and were represented on the lowest horizontal plane in the cube design. The ‘‘centroid’’ (37uC, pH 7 and 2.5% added NaCl) sample represents the mid-point of the variable parameters and was essential ... optimal adaptations for survival in changing environments. Citation: Crompton MJ, Dunstan RH, Macdonald MM, Gottfries J, von Eiff C, et al. (2014) Small Changes in Environmental Parameters Lead to Alterations in Antibiotic Resistance, Cell Morphology and Membrane Fatty Acid Composition in Staphylococcus lugdunensis. PLoS ONE 9(4): e92296. doi:10.1371/journal.pone. ... conditions involving temperature, pH, nutrient and water availability as well as exposure to toxic chemicals. Once colonized on the skin surface as a commensal, the environmental parameters of temperature, pH, osmotic pressure and water availability would be more stable. In the event of a wound breaking the skin surface, the bacterium must go through a
EFFECTS OF SILVER NANOPARTICLES IN SOLUTION AND LIPOSOMAL FORM ON SOME BLOOD PARAMETERS IN FEMALE RABBITS DURING FERTILIZATION AND EARLY EMBRYONIC DEVELOPMENT
... organism and its effect on various biochemical processes have not been clarified definitively. The main purpose of this study was to investigate the influence of subcutaneous injections of silver nanoparticles in solution and in liposomal form (as targets of macrophages (Kelly et al., 2011) on hematological and biochemical blood parameters of New Zealand White ... 274-278 There were no significant differences in biochemical values of blood among the groups (Tab 4), and no significant differences in the ratio of globulins fractions (Tab 5). Silver ions show a high affinity for the thiol group in the liver that can lead to hepatotoxicity (Drake, 2005). Our results of biochemical parameters in the silver group are similar ... administration than that in the CG. There were no significant changes in red blood cells parameters, platelets, and activity of some ferments (ALP, AST, ALT, LDH, GGT) between control and silver groups during the entire period of experiment. In conclusion, the hematological and biochemical values of blood obtained in the given study showed that free silver nanoparticles
ACCUMULATION OF SELECTED METALS IN UMBILICAL CORD BLOOD OF NULLIPAROUS AND MULTIPAROUS WOMEN AND CORRELATION WITH THE NEWBORN´S PARAMETERS
... (1) 358-367 parameters. Parity influences the concentration of cadmium in umbilical cord blood with higher level found in multiparous women. Average content of iron in cord blood did not decrease with parity, it indicate that this element is preferentially taken up by the child. Keywords: cord blood, magnesium, iron, copper, cadmium, parameters of ... circumference of a newborn. 361 JMBFS / Kozikowska et al. 2012: 2 (1) 358-367 Table 1 Characteristics of the newborn’s parameters depending on the number of pregnancies (multiparous n=46, nulliparous n=53) Newborn’s parameters Multiparous Mean Min Max Std.Err Mean Nulliparous Min Max Std.Err Birth weight (g) 3084.13 1400.00 4250.00 ... et al. 2012: 2 (1) 358-367 REGULAR ARTICLE ACCUMULATION OF SELECTED METALS IN UMBILICAL CORD BLOOD OF NULLIPAROUS AND MULTIPAROUS WOMEN AND CORRELATION WITH THE NEWBORN´S PARAMETERS Iwona Kozikowska*1, Katarzyna Suprewicz1, Katarzyna Forma2, Katarzyna Miszczuk2, Anna Piekarz3, Jerzy Sikora3 Address: 1Pedagogical University of Cracow, Department
Biochemical changes in soybean leaves infected by Corynespora cassiicola
... of leaf area was counted at five randomized places on on the 8th trifoliate leaves from the plants of each replication and cultivars with a hand-held microscope. Biochemical analysis For all biochemical assays, the 6th, 7th, 8th and 9th trifoliate leaves, from base to the top, of plants from the replications of each treatment (cultivars × plant inoculation) ... randomized design was used to obtain the samples for the biochemical analysis. For severity, the ANOVA was considered a 2 × 4 factorial experiment consisting of two cultivars and four evaluation times (4, 6, 8 and 10 dai). For NLL and AUDPC, the ANOVA was considered a one-way ANOVA consisting of two cultivars. For the biochemical variables, the ANOVA was considered ... procedure........................................................................................................... 42 Disease assessment............................................................................................................... 43 Biochemical assay ................................................................................................................ 43 Determination of defense enzyme activities
DETERMINATION OF INTERSECTION CROSSING TIME DEPENDENCE FROM THE AMOUNT OF VEHICLES AND ROAD NET PARAMETERS
Biochemical identification of the leishmanias
... classification is not always clear, however, if the determination is based on experimental sandfly infections (2, II). Therefore, an evident need exists to exploit further parameters for taxonomic purposes -parameters that do not depend on complex interactions between the parasite and a vector or mammalian host. This is particularly true in cases where correct medical ... work of Lanotte et al. (26). Both methods produced similar dendrograms that clearly delimited L. major, L. tropica, L. aethiopica, and L. donovani. Other Biochemical Methods There is a wide range of other biochemical procedures that are useful in taxonomic studies of leishmanias. With the possible exception of procedures using genetic probes and lectins ... WHO 56467-480, 1978. (19) Tait, A. Evidence for diploidy and mating in trypanosomes. Nature 287:536-538, 1980. (20) Miles, M. A. Leishmania: Culture and biochemical comparisons-some difficulties. In: UNDP/ WORLD BANK/WHO. Biochemical Characterization of Leishmania. Geneva, 1982, pp. 123-137. (21) Shaw, J. J., and R. Lainson. The in vitro cultivation of members