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  6, MPs participate in both amplifi cation of infl ammation atand in each case the number of (A) necrotic myofi bers, (B) phagocytedthe time of injury and down-regulation of the infl ammatorymyofi bers, (C) regenerating basic myofi bers, and (D) centrally nucleatedresponse to avoid excess tissue damage (31, 53, 54). Notexin (Nx) was injected into the TA of CD11b-DTR mice at day 0, and DT was injected in the same muscle at day 5 (A) and days 5 and 6 (B and C).(A) 24 h after a single i.m.

MATERIALS AND METHODS

  Because of high inter- individual variations (3–12% of PBMCs), the number of circulating MOs incontrol mice was normalized to 5% of PBMCs for each series (mean calcu- lated with >25 mice). mpcs were plated on previously prepared MP cultures (3:1 MP/mpc ratio) in advanced RPMI 1640 supplemented medium, except in some experiments in which MPs and mpcs were seeded together before MPtreatment was applied, as described in Human MP cell culture.

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