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Fish Culture

Three sympatric karyomorphs in the fish Astyanax fasciatus (Teleostei, Characidae) do not seem to hybridize in natural populations

... (Figs 2 b, d, f, details). FISH analysis, however, showed the presence of 18S rDNA at the distal regions of five chromosome pairs, but no FISH signal was observed on the short arm of any chromosome (Fig. 2 b, d, f ). This points towards the presence of a small number of 18S rRNA genes in the short arm of the sm chromosome. Double FISH showed the presence ... Neotropical fishes: Methods, applications and perspectives. Journal of Fish Biology 76: 1094–1116. doi: 10.1111/j.10958649.2010.02564.x Vicari MR, Pistune HFM, Castro JP, Almeida MC, Bertollo LAC, Moreira-Filho O, Camacho JPM, Artoni RF (2010) New insights on the origin of B chromosomes in Astyanax scabripinnis obtained by chromosome painting and FISH. Genetica ... staining technique (Howell and Black 1980). Mapping of the ribosomal DNA (rDNA) was performed by fluorescent in situ hybridization (FISH) according to Pinkel et al. (1986). The 18S and 5S rDNA probes were obtained from the fish Prochilodus argenteus Spix and Agassiz, 1829 (Hatanaka and Galetti Jr. 2004) and Leporinus elongatus Valenciennes, 1850 (Martins
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Incidence of Aeromonas spp. infection in fish and chicken meat and its related public health hazards: A review

... the fish and shrimps [20]. One reportof isolation of a total of 319 strains of A. hydrophila from 536 fish and 278 prawns for a 2-year period survey and the samples were collected from a major fish market of Coimbatore, Tamil Nadu, South India [18]. There was the detection of 82 strains of presumptive Aeromonas spp. out of a total of 250 frozen fish ... zoonotic importance of Aeromonas pathogen in fish and chicken meat. Keywords: Aeromonas, antibiotic resistance, chicken, fish, public health. Introduction In the last two to three decades Aeromonas spp. have emerged as an important human pathogen.Praveen et al. [1] conducted a study on 179 samples from fish (Gills), and chicken (Raw meat) were processed ... examination. Ampicillin dextrin agar (ADA) media was used for isolation of aeromonads from fish and chicken. A total of 31 (17.32%) isolates were obtained from 179 samples of fish and chicken in the North Kolkata Region. In 31 isolates, the highest isolation were achieved from fish 24 (18.89%), followed by chicken 7 (13.46%) out of which 29 (93.54%), 1 (3.22%),
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Elimination of Enhanced Thermal Resistance of Spheroid Culture Model of Prostate Carcinoma Cell Line by Inhibitors of Hsp70 Induction

... (JABEREBN-HAYAN). Monolayer culture DU145 cells were cultured as a monolayer at a density of 104 cells/cm2 in T-25 tissue culture flasks (Nunc). The cultures were maintained at 37°C in a humidified atmosphere of 7.5% CO2. Cultures were propagated or cells were harvested by trypsinzing cultures with 0.5 mM EDTA/ 0.25% (w/v) trypsin in phosphate buffer saline (PBS). Spheroid culture Spheroids ... 37°C humidified atmosphere with 7.5% CO2. Half of the culture medium was replaced with fresh medium twice per week. Heat and quercetin treatment of monolayer cultures 5×105 cells per flask were cultured in T-25 culture flasks containing RPMI-1640 culture medium supplemented with 10% FCS for monolayer culture. After 48 hours, they were treated with 50 μM quercetin ... DU145 tumor spheroid cultures as compared to the prostate carcinoma cell line's monolayer cultures. Materials and Methods: DU145 cells were cultured either as spheroids or monolayers. Cultures were treated with hyperthermia in a precision water bath (at 43°C for 60 minutes) and/or quercetin (50 and 500 μM for monolayer and spheroid cultures respectively).
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Nutritional evaluation of commercially important fish species of Lakshadweep archipelago, India.

... on fish lipids, fatty acids [7] and amino acids. However, a big variation has been noticed in these compositions of different individuals of the same fish species. Researchers have found that freshwater fish contain lower proportions of v-3 PUFA than the marine fish [8]. Therefore, the ratio of total v3–v6 fatty acids is much higher in the marine fish ... v-3 and v-6 PUFA in the present fish samples is in good agreement with the findings of Huynh and Kitts [7]. The saturated and monounsaturated fatty acids are generally abundant in fish from warm or temperate regions, whereas PUFAs show higher levels in fish from cold regions [35]. Compared with freshwater fish, marine fish have higher levels of PUFAs, ... Hence, the fishes of Lakshadweep Sea are highly recommended for consumption, since these fishes are highly enriched with nutrition. The results can be used as a baseline data for comparing the various nutritional profiles of fishes in future. Citation: Dhaneesh KV, Noushad KM, Ajith Kumar TT (2012) Nutritional Evaluation of Commercially Important Fish Species
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Effets de la vitesse et de la durée du remplissage du grain ainsi que de l'accumulation des assimilats de la tige dans l'élaboration du rendement du blé dur (Triticum durum Desf.) dans les conditions de culture des hautesplaines orientales d'Algérie

... la durée du remplissage du grain ainsi que de l’accumulation des assimilats de la tige dans l’élaboration du rendement du blé dur (Triticum durum Desf.) dans les conditions de culture des hautes plaines orientales d’Algérie Fayçal Bahlouli (1), Hamenna Bouzerzour (2), Ammar Benmahammed (2) (1) (2) Université Mohamed Boudiaf. Faculté des Sciences. 28 ... remplissage, Algérie. Effects of speed and the duration of grain filling and the accumulation of assimilates of the stem in developing the durum wheat yield (Triticum durum Desf.) in the culture conditions of the high plains of eastern Algeria. The present study was led on the experimental site of station ITGC in Setif. The objective is to determine the differences ... stem decreases when the environment allows the expression of a better grain yield. Keywords. Wheat, assimilates, duration of filling, yield, speed of filling, Algeria. 1. INTRODUCTION La culture du blé dans les régions semi-arides est soumise à différentes contraintes abiotiques qui minimisent de façon très significative le rendement en grains. Le transfert des
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Maintenance and methods of forming theoretical knowledge and methodical and practical abilities in area of physical culture for students, future specialists on social work

... on discipline the «Physical culture? ? assist the certain affecting depth and breadth of mastering of knowledge of professional preparation. теоретичні, знання, методичні, уміння, навички, фізична, активність, культура, студенти, соціальна, робота. theoretical, knowledge, methodical, abilities, skills, physical, activity, culture, students, social, work. путей ... широту засвоєння знань професійної підготовки. Leyfa A.V. Maintenance and methods of forming theoretical knowledge and methodical and practical abilities in area of physical culture for students, future specialists on social work. The value of theoretical knowledge, methodical, practical studies, skills in forming physical activity of students is rotined. The
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THE NAMES AND MARKINGS GIVEN TO THE HORSES ACCORDING THEIR COAT COLORS IN TURKISH CULTURE <br> TÜRK KÜLTÜRÜNDE DONLARINA GÖRE ATLARA VERİLEN ADLAR VE NİŞANLARI

... History of Turkish or Turkic Volume 4/8 Fall 2009 Türk Kültüründe Donlarına Göre... 1831 THE NAMES AND MARKINGS GIVEN TO THE HORSES ACCORDING THEIR COAT COLORS IN TURKISH CULTURE ABSTRACT One of the richnesses of the Turkish language is the coat colors and markings of the horses. However, it’s not possible to talk about a similarity while giving
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Epidemic and Endemic Malaria Transmission Related to Fish Farming Ponds in the Amazon Frontier.

... available, hectares of aquaculture in Mâncio Lima were estimated based on fish yield as calculated in the neighboring state of Amazônia (1_hectare of aquaculture constructed per year = 70 kg of fish) [24]. We used the reported fish harvest production data [25] and this transformation factor to estimate the number of hectares of aquaculture constructed per ... in Fig 5. A map of fish ponds and malaria cases in the Mâncio Lima town in 2011 and 2012 is shown in Fig 6. Buffer zones around fish ponds are also shown. Fish farming in Mâncio Lima is mostly carried out in small farms or in the backyards. These ponds were constructed by digging or by damming small streams. It is common to find fish ponds close to ... cohesion (%). To compare malaria incidence and fish pond construction between strata, we used ANOVA and Wilcoxon’s matched pair tests. In Mâncio Lima, where primary data was collected, a map with the location of all malaria cases in 2011 and 2012, as well as the fish ponds, was created. Buffers 100 to 1000m around fish ponds were constructed and the number
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The seascape of demersal fish nursery areas in the North Mediterranean Sea, a first step towards the implementation of spatial planning for trawl fisheries.

... assumptions: (1) juvenile fish are particularly vulnerable to a fine mesh trawl fishery [12], especially when they are concentrated in nursery areas, and (2) a PLOS ONE | DOI:10.1371/journal.pone.0119590 March 18, 2015 2 / 25 Fish Nursery Areas in the North Mediterranean Sea reduction of fishing mortality on immature fish represents a fundamental ... impact of fishing on the exploited populations and ecosystems. The reduction in juvenile mortality is particularly relevant in the Mediterranean and is considered as one of the main prerequisites for the future sustainability of trawl fisheries. The distribution of nursery areas of 11 important commercial species of demersal fish and shellfish was analysed in ... analysed the spatial distribution of nurseries of some of the main commercial species of demersal fish and shellfish exploited in the EU Mediterranean basin, using the longest existing time series of standardized abundance data collected on demersal fish and shellfish in the North Mediterranean Sea (i.e MEDITS trawl survey, [29]) as one of the main outcomes
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Influence of culture is on development of corporations (on the example of corporations of the USA, Japan, countries of Western Europe)

... корпоративних відносин, управління персоналом. Igor Stoyko, Vasyl Getscko INFLUENCE OF CULTURE IS ON DEVELOPMENT OF CORPORATIONS (on the example of corporations of the USA, Japan, countries of Western Europe) Abstract. The comparative analysis of features of elements of economic culture of the USA, Japan, countries of Western Europe is given. Generalized experience ... с. 4. Управление качеством продукции и освоение передовой технологии (Опыт США и Японии) / Конарева Л.А. / ЭКО. – 2000. – с.155-170. 5. www.henkel.ua/careers/henkel-corporate -culture- 1442.htm. 6. www.diplomukr.com.ua/news/2009/10/16/1395. 118
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Selenium Supplementation in Fish: A Combined Chemical and Biomolecular Study to Understand Sel-Plex Assimilation and Impact on Selenoproteome Expression in Rainbow Trout (Oncorhynchus mykiss).

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A subset of osteoblasts expressing high endogenous levels of PPARgamma switches fate to adipocytes in the rat calvaria cell culture model.

... from 12, 15, 17, and 21 days of culture were collected in each of two independent experiments; a portion of cells of each colony was re-plated at high cell density with BRL, while the remainder was collected for total RNA preparation. One hundredfifteen and 132 colonies in each experiment were successfully adapted to subculture, and of these, 94 and 95 ... double negative ALP2/oil red O2 are neither osteo- nor adipogenic (16/97 and 16/95) (Table 1 and Fig. 1K). Notably, colonies picked and subcultured at day 12 exhibited mainly monopotential adipogenic fate at subculture, while colonies picked on day 21 were mainly monopotential but for osteogenic fate. Double-positive colonies/ bipotential fate occurred ... to those without BRL up to day 5–6, and thereafter adipocytes were seen within cell condensations (Fig. S1). In contrast to what was seen in BRL-treated cultures, a very few adipocytes were dispersed in control cultures without BRL, but they disappeared as ObL progression occurred (data not shown). Likewise, colony formation assays revealed that there were
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Baseline predictors of sputum culture conversion in pulmonary tuberculosis: importance of cavities, smoking, time to detection and W-Beijing genotype.

... Time to detection (TTD) on automated liquid mycobacterial cultures reflects mycobacterial load more accurately than sputum smear or culture grading, and has been shown to predict month 2 culture conversion, tuberculosis recurrence and relapse [3,4]. Important factors associated with smear and culture nonconversion after 2 months include increasing age, ... cultures were confirmed as M. tuberculosis complex using an in-house PCR assay [21]. The date of culture conversion was taken as the date of the first negative culture provided there were no subsequent positive cultures. Participants whose first negative culture occurred at week 8, were regarded as converters. Routine phenotypic drug susceptibility testing ... were lost-to follow-up and by week 8, this increased to 15%. Two cultures from participants who had not undergone culture conversion up to week 7, had contaminated cultures at week 8 and were categorized as not having converted. On Kaplan Meier analysis 50.4% of participants had undergone culture conversion by 8 weeks (data not shown). was not significant
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The Comprehension Process of Audit and Accounting Culture through the Academic Environment

... influence 2 9 < 0 , 6 5 2 9 2 5 1 6 2 + / 9 3 . 1 2 . 6 0 5 < > : 7 5 : > 3 , 9 5 9 6 3 6 0 , 0 . 5 ? , 5 9 8 3 9 2 J 6 / 0 0 , + ? / 2 1 / 5 , 0 , 9 6 3 1 , < 3 . 2 : / 3 3 + , 5 9 0 . I 3 3 I 6 9 . 8 : 0 8 5 , , . 0 6 + 5 i 6 J I , 3 6 O 3 6 Ţurlea M / P 9 / ; 3 > 2 5 ? X P 2 , 0 3 U 3 3 O , . f D 2 5 2 + 6 , 3 0 0 > 5 2 5 L . J , 6 9 0 6 . : c > 2 ` , L 0 5 , , + a M g , 0 3 J M > 1 3 + 0 J > 2 _ 8 > 3 ? , 5 Y 9 + 5 , 5 F 3 0 9 , 5 : 0 / 3 < 1 , 0 6 6 , 0 3 > P 3 3 9 3 1 O 2 2 , , / , 5 1 I / 0 / , 0 5 1 5 > , 5 3 3 \ 1 1 5 > . L . E 9 5 9 I 5 7 U 5 9 9 : I D . , ? 7 g j L 5 : 5 9 5 P , 0 , 9 . + 1 < b 9 3 , , / > O 1 / , 2 6 1 8 0 O 5 2 6 I , 9 . 3 6 + I 0 ` I I 6 6 0 0 I 9 5 . 3 9 , 3 8 < 6 0 3 I 3 5 : : 1 , / , 9 ? 3 5 / 3 5 5 , 3 3 9 6 5 9 < 5 5 9 2 _ 6 6 L 3 5 M 3 < 2 9 < : , 0 1 , 0 3 2 , < K < 6 , 5 L 6 1 7 9 9 5 9 6 , I 0 M 5 9 3 + 0 : 5 3 0 + , @ 2 9 0 < < L 0 5 I 6 , 0 O 5 , / 6 1 3 , 8 / M 9 5 2 6 > , 1 , > 0 2 The comprehension process of audit and accounting culture? ??………… _ O , O 9 L 1 , 0 1 1 R 9 I 6 : , 5 I 5 @ 2 2 + 0 / 3 3 9 9 5 + , 2 3 , 5 < 6 L 0 5 O P 5 9 , 6 0 9 0 2 I 1 0 , , O L , , 9 2 9 1 ? . 3 0 I 0 5 2 3 , O > P I , , < O , 0 , 0 5 2 5 3 1 3 , , 9 > J : 3 0 1 9 9 > . , 6 5 1 2 3 1 0 J , P 3 I + 2 6 , 2 0 1 + , / + 0 . + 9 > 6 2 O 6 3 3 3 0 + + , 5 0 6 3 1 3 0 > 3 3 + + 3 , 6 2 3 , . 3 > 0 . > , / / , 6 9 5 5 . l 3 3 5 3 2 + 6 , 5 J M + , / , 1 5 + < 0 6 O 3 9 J * 3 9 d 3 P < 3 , Q , 0 / 3 3 3 6 5 , > 5 , 2 6 7 9 > / , , > 9 2 2 0 . . I 2 3 1 6 > , P 5 P 3 I : 6 2 J 1 L 3 0 3 , 9 + + , 9 6 + 6 / , 5 3 1 5 > , 2 9 . 3 . , / 0 9 2 , / > , 5 0 3 6 6 9 3 1 9 5 0 / 0 P 0 3 + 5 3 I , + 1 , 3 5 , , O , 7 O 0 O 6 9 1 3 5 R 6 1 5 9 1 , , < 5 , 0 , , J P + 5 L 3 I 2 L 1 0 0 , 9 / 5 / > 0 3 > , 6 3 5 / 3 , + 2 9 2 , 0 + : 5 9 0 > / 3 O + 3 + , 9 O M q * 8 , 0 1 ? P + 8 , 2 > , I ^ : 6 5 L 5 5 9 0 E , 3 6 2 9 2 : I 6 , 9 D / 5 1 3 6 6 0 , , I 2 3 + 9 9 3 3 3 , > . 0 > O , / , , 3 5 3 3 , 9 + 2 , : 5 2 3 1 6 5 : 3 1 P 9 , J < . 3 J , 6 P J 0 6 8 9 5 5 9 5 3 0 6 + 5 J , 9 > 9 5 . : / 3 0 + 3 + 6 3 + 5 , + , 9 e 5 6 < 3 / 0 n I I e / 8 3 0 / 2 / 3 + I . 1 , / 0 2 > , < 6 / M 0 9 8 3 5 , 2 J 8 , 0 + 6 , 2 * 3 2 , + , , < 6 > 3 + , M 2 / 5 9 3 3 < 2 5 9 ? , > 5 5 3 J , 6 0 > 1 2 6 / , 9 3 7 5 3 < 5 3 6 0 2 . 5 2 I J 9 + 9 2 , , 5 / ? / , 1 6 / : 8 1 5 , 0 9 3 . 0 7 1 6 . 2 , L 7 a . , + 6 9 9 2 : 3 3 M / 1 6 / 8 0 0 , 9 > 0 0 7 . 5 L , 5 0 6 < 3 3 > 0 9 2 6 0 > : < 3 J < 5 9 , 0 5 1 / 2 3 K 6 6 > , 2 6 3 , , / 8 5 + K 5 5 . 3 I 2 1 9 , 0 J 9 / 7 5 6 , / 0 0 < 3 9 0 + 5 , . 2 5 , 6 + , < + , 3 < 3 + 3 + > / 1 , 3 : , , 0 : P : 9 / , , / W 5 2 / 5 , 2 U 9 5 , + 6 3 , . 3 > . I I < u + 5 I 9 ^ 3 3 , : : / 9 < 5 5 9 > U , P 3 / 5 E 5 2 0 3 I / 2 0 E 5 0 2 0 0 J ] 9 ? + I 5 O 5 U 2 3 , . 0 6 F , > I 2 O 5 , 0 2 1 + 6 / : / 0 , 0 1 : 6 > 3 3 U * , / D t P 0 , 2 7 , 0 ? I . 8 > 6 3 r L 1 9 : , 2 2 6 9 5 o N 6 q j / + 2 3 5 6 + J , L 9 0 0 3 5 : 0 3 + 3 0 9 3 5 6 . / I ? e 6 : 6 > n n p 6 5 2 6 M . 3 6 < L 0 > I 0 6 8 . 5 / 0 3 6 9 5 1 0 9 / 2 : / 3 , 9 0 2 . 3 , 2 2 6 5 9 3 < 6 , - 5 0 / 5 J 5 1 , , 9 5 1 3 8 9 L 0 . 3 6 9 > 0 5 . 1 , 1 5 , 3 2 2 > 3 ? : 0 2 6 9 I 3 1 6 M o 3 J . e 1 3 : 2 3 9 1 0 I / 6 + / 6 9 2 / 5 3 6 5 > 3 + 0 / 3 9 > 2 3 6 2 : : 0 q 2 , 2 , 1 2 , 9 < L 5 / / 6 + , 0 3 0 2 , 2 6 6 K 5 > , : O 6 6 3 > J 0 5 0 : + 8 5 J 9 . I : I 3 = / + 6 9 6 2 9 8 2 > 6 2 , 6 5 R 0 6 3 I J + ? 0 6 5 3 , > 3 + 3 2 8 5 > . I * 0 5 + 0 + 5 P / M / 0 3 6 2 1 2 J 3 3 3 0 0 2 6 0 6 2 < I 2 . < S , 5 0 , 0 , 1 9 5 0 1 J 2 6 / > 5 5 0 1 5 6 9 > , + , , 3 6 2 2 < , q p J 3 + P / 5 3 6 / L , P O 3 1 9 , + L 2 6 , 3 > 9 1 3 6 2 , 1 7 + 3 : : , + , > ? , 1 3 L , 5 1 + , 9 3 0 P 5 I , , , 0 0 2 I I 3 , 1 , 1 , > 0 L 6 0 9 : : , 1 3 6 > 5 , / 9 > . 9 2 9 5 3 : 3 : 7 6 5 + 0 J 0 , 1 7 6 9 5 , . > 1 0 0 2 : / 6 6 5 . 3 > 6 8 0 3 9 5 / : 6 0 I M 5 1 / < q _ 3 , 6 , 5 0 < 5 7 . 1 > 9 0 9 , 5 5 , / L 6 9 > / 9 J . 5 0 , > 5 0 + , 3 > + 9 5 0 / 6 3 3 6 . L 5 , 2 < 0 + 9 < , . 1 O 6 , 1 , 5 2 7 , / 2 6 0 6 3 / n . + 3 0 + M 2 5 1 I 9 3 + 5 0 q _ L 3 6 3 5 L : 6 > , , 9 , < 3 + 5 < , 0 3 5 5 2 2 2 6 9 3 ? > L 1 6 5 9 1 6 + ` 9 + , 7 3 2 6 3 > 5 6 9 0 , O 5 , O K 3 9 . 2 < 0 5 K 3 1 5 , / 9 , 5 0 0 + 3 5 7 J 3 . , : 1 5 I 6 < 9 9 5 6 0 2 , 5 J 3 6 . J : 6 6 2 / N P 0 9 2 2 8 I ? , 5 9 > , + 0 5 6 0 + / 9 0 m 3 < 6 6 0 : / I 2 7 > 6 L 5 k 9 6 L 0 5 > 3 0 S 5 , 5 , , 6 0 / + r 5 v , J 5 O L / , 1 2 6 0 M / 2 o 3 a 6 9 e / , 1 q n p 2 , P S 0 , ? 3 < 1 , O 2 , L I , , , 0 1 5 ? / / w + 9 + + . 9 0 5 6 3 0 2 1 5 2 6 , + 9 2 > O + v 5 0 , 7 7 6 , / + q o k k p L 1 9 7 6 > , , 7 6 > , 5 / , 3 + 0 3 0 / / 9 . 5 3 6 5 J > 6 7 , 1 2 6 3 8 / 0 5 0 : : , / 3 3 + , 0 / 3 6 9 5 2 q 9 : 0 I 6 / 6 5 / 2 > 9 3 6 6 . 5 7 5 J 6 3 2 > 6 . 5 9 0 I J 5 6 > 2 6 3 9 5 7 7 , / , 1 R 2 2 3 6 , 3 9 1 2 8 S x / / + O 0 0 + 5 5 6 0 J , I , > 2 = 7 ? 0 , , 1 I . 2 , , < > 0 I J 0 8 , 0 5 3 1 : 2 0 : 5 , 3 0 / + 3 0 5 3 3 > + + - 6 , 0 5 7 , > . > I < 0 , o P / , 1 . k 1 k 0 / 6 0 5 I m p > 2 7 6 < + 9 9 L I 0 O . / 3 < 3 + 0 , . 3 9 L 9 : 5 d 3 y a + , 0 . q o e < 0 9 2 . > + 0 5 9 3 , I , < , 5 3 2 M N 9 9 2 P 3 1 1 , , ? / _ , M 5 3 I a 8 c ? 9 0 L 0 2 ? 2 ; + Ţurlea M 9 O 5 P 8 = 0 I 3 , 1 0 0 5 > , 7 , z 5 I 7 ? 3 2 0 1 3 1 5 2 1 0 3 8 / 0 5 3 6 < 0 3 0 I 9 0 , 3 , / 0 / : 5 + : , . > 5 / 3 3 6 3 > 0 3 . , 9 0 9 . 5 3 6 5 > : : 1 L 9 , 5 5 5 1 / , / > , 1 > . < 0 , o 6 0 9 . 8 2 > : I 0 , 0 , / @ , J + 9 5 3 > / 0 n 6 9 9 3 y : 3 , 3 n 5 6 5 + e + 5 . 3 : , p 2 2 ? 0 5 / / 9 < , , _ / : 2 6 0 1 5 0 3 5 . 1 , I 1 3 0 0 5 6 I L 6 2 M 5 / 0 9 3 3 J 9 6 5 J 0 O 0 8 M w + 0 3 + 0 < , 3 0 I M o e > H 9 I 5 6 K , 1 ? o e n n l p ? > | _ 1 / 8 ? o q P 8 > = 6 : : O : , : / 0 , I 1 3 , , 5 1 0 / , , 1 0 2 5 6 > v 5 3 + , O , : 6 6 2 5 o 0 5 e / e 6 p 0 6 2 5 p 0 5 2 > / 2 , n 2 , 3 1 n 3 / . 0 > , 5 6 2 , 0 2 1 , n p ? v , O 6 2 0 5 > = 0 I 3 , 1 o e n n p ? { 9 5 , 2 q n p ? v 0 1 2 9 5 } z , 5 5 8 ? o k k k p ? 0 I I / 6 3 , > q n 6 n , 1 5 , e r n q 5 , o 6 3 / 6 2 5 , > . / . @ J 5 1 / 3 3 6 L 5 2 1 c J , 8 9 > 5 , 0 , 3 O 5 1 0 9 , 2 6 + L P 6 / J , 2 6 0 P I , . 6 + q 0 v q p , , 6 b q n > 1 > M I 1 ? , 2 L 9 + 1 9 3 6 O 5 3 J L + 0 1 0 3 / 3 3 6 + / , , 1 , 2 0 P , 3 1 , O 2 , , 3 6 I 5 I 3 2 + 6 , J 5 / 6 9 : . 6 5 / 3 0 1 5 6 3 , 2 M q N 9 1 , 7 7 I , , , 0 1 1 0 . 7 9 I , , 7 , > L I , c 3 2 0 ? 1 + L , 9 > 9 6 7 P , 6 8 / < > 3 2 L , + . v 9 > 1 3 P , 0 M 0 6 3 0 / 3 > ~ 5 8 . ? 5 , + 9 , 3 , / 0 : 0 0 , L / < f / M 1 9 =  9 L . 6 , , 5 3 < 6 J , 1 6 I 1 , 5 0 2 / 7 J 0 L 1 6 > . I 1 , N . 0 0 / 0 M 3 3 O 6 6 9 3 0 b _ 5 6 5 > 5 9 9 2 ? , , : 1 7 6 3 2 I 9 0 + f c , 0 , / / 5 , 3 > L 9 > L 9 < + P 0 ? _ L 0 , 6 8 = 5 5 3 M e 8 : 0 o 0 9 5 n 1 6 I n 2 < 3 0 0 ? 3 + 6 € p , 9 5 ? _ ? q c 9 1 I L , 0 L 0 ? 9 1 _ 1 J 3 M 6 , 5 a J @ , : / I / 9 , S 9 6 . O 6 9 < . 6 0 5 I I 3 / 3 1 6 8 8 9 , 6 2 0 _ 0 / 1 / , 9 I 0 / / 9 . 0 3 / 9 , 3 / 6 5 3 9 6 I , J 3 8 7 , / / < 9 , . 0 5 2 3 . 1 + 6 2 : J , 5 1 1 2 5 3 6 3 6 0 < < 0 3 0 1 > > 6 6 2 / I 9 , 9 0 0 6 < 5 3 L : + 9 0 1 , 1 j * > 2 2 9 , 6 . 3 @ 0 6 1 5 a 2 O L , 2 > , : 0 5 5 I : 6 K 2 , 3 = R 3 3 0 < 6 5 0 P . + 9 3 6 j + 5 0 0 9 , o 3 P 0 I L , 1 > 6 1 5 6 n 3 9 , L 7 5 , , I I 6 + 0 5 6 9 3 . 0 / L > / J 0 p 5 6 5 y 0 0 3 : n = L 5 > e @ : . 2 2 a 9 / 0 6 5 2 / 1 : 9 L 6 5 < 0 , 5 5 / 3 6 0 2 I 9 : 0 1 6 @ . 0 I 6 < 3 3 0 0 1 8 1 3 , 5 0 / 2 + 0 > 0 3 3 : 5 L 5 3 9 3 3 , + 8 2 6 , 3 = > 3 . P a 0 3 9 0 j 2 3 , > I 5 6 > 2 / , 2 , / + , P 6  6 1 6 3 0 L 1 + 0 0 1 8 8 + / c I 0 9 2 5 1 9 6 + 6 2 L , 3 2 9 9 3 , , w / 1 : x 1 K 9 I 6 6 0 3 3 P 1 0 7 > > L 5 1 , , , , 2 0 8 5 2 6 + / 3 6 L , 1 , , 9 I . 2 5 L 0 2 1 + 1 7 , 7 6 I 7 < 3 + 0 < 2 3 2 . , 6 3 9 3 3 9 0 5 3 . 3 < I 6 1 3 6 5 9 9 0 L 6 6 : 2 2 3 2 2 . 0 , 9 6 5 + 1 2 8 < x M 3 , 0 3 / . € O 1 6 ? P ? . S p > 2 3 , d I , I I n . . . : n 9 I / 8 0 1 e / P / 2 6 0 P 6 0 2 I 0 , 7 . L 3 + R 3 o 1 5 , 3 5 + . . , 9 . > 1 3 8 M 2 0 3 6 > J 2 , 0 > L / 3 + < 5 9 , 2 5 1 , 9 5 3 , L O 0 5 6 7 2 3 > > 6 3 1 L / , I 3 . / 6 . 5 8 5 6 2 9 . 5 / 1 / . . 3 9 a 3 9 0 1 . 6 / J 3 3 : 9 0 5 5 0 M 1 > 8 > / > 9 2 . 0 5 : , 5 , 6 1 0 1 0 5 . P 6 p 2 0 5 0 6 , b 3 . , 9 3 ? 0 3 e 6 , 3 I 5 > . . / O 5 9 J 4 * 0 : 5 x / 0 9 9 5 5 , n 5 P 9 J 9 0 9 1 e 0 5 L , 5 + 7 < 1 3 , o L 0 , 7 9 2 I < 3 M < / 9 5 1 , 9 3 , < 2 5 5 1 1 3 9 . 5 = 5 6 6 . = 1 / / 6 ? 2 , 0 5 2 5 , / 3 0 1 0 : 9 5 < I 1 9 3 / 9 3 L , 6 6 9 2 : 5 + < I : < , P j 9 9 H P ? 1 3 6 , 9 / 3 , 9 + , ? : 0 O 0 / + / . 6 9 I . 3 + 9 , 3 5 3 6 1 + 5 6 3 8 0 , J 2 6 5 , : 5 / 9 > 9 1 , 5 < 5 : > 0 3 3 6 9 9 1 O / , 9 5 < 1 0 3 , , / I 9 5 0 . 3 3 5 9 , 3 : 3 1 8 3 9 + 3 I , 7 , + , , I M In ... the , 6 5 3 1 3 8 ‚ 0 + , 1 6 6 5 3 9 + 5 8 9 5 9 L . I 6 3 9 / J / J 2 / 6 0 9 2 6 5 3 6 > + 6 5 9 J , 9 3 O 6 > 0 O 9 + 5 , < : . 5 9 2 O 3 / > > 0 + 0 f 6 3 1 0 6 9 6 5 5 3 + 6 5 3 . 3 O 3 , 6 > 3 5 7 2 + , 3 * + 5 6 : , , O 9 , : 3  + 9 : , 2 3 1 , + 9 8 L 5 M 6 2 I 2 > 9 1 5 6 , 1 6 2 9 3 9 , / 3 0 2 , / 6 3 L 0 < 2 6 > P , : : , 0 2 9 > , 2 . 9 0 5 6 , 2 I 0 5 P 9 9 I 0 : 8 , 9 . 0 6 3 , I / / < , + / 5 5 6 , 1 I 2 6 3 , 6 J . 3 3 8 6 , . 1 9 0 1 + , , L > L 8 2 + / , P 9 2 , 5 > L / 5 9 < > 9 3 > 0 9 6 5 / 9 : , 3 0 , 9 + 7 5 8 : 6 2 9 I > 9 5 2 6 , 6 < , 6 2 3 2 3 6 9 5 1 6 . I 5 ? , 5 M 9 2 O 0 9 9 5 0 + : 5 , > 1 , The comprehension process of audit and accounting culture? ??………… d m they “attempt to usurp, exclude and establish monopoly over the field’s reproduction and type of power effective in it” (Everett, 2002). * 3 9 3 + , > 3 + 1 6 2 J . > 9 6 . + 6 2 J , 2 3 , , 1 ? 6 + 0 P 3 > 5 . + . 3 / 0 > 6 3 9 , 3 + 9 / 6 0 2 9 9 5 . 5 2 6 I , : 0 I J L I I 2 6 9 8 8 3 2 6 < 6 , 5 , < O . 3 1 , 0 K , ? 2 7 6 0 , 5 6 5 5 5 > 0 L I I 9 2 < < > 0 3 9 1 / 6 0 1 7 3 , 2 I 9 > , 6 6 9 , . J 5 P , 5 1 5 3 I , 2 9 9 O O : 2 P 0 + / 6 + 6 6 3 8 / 9 I 8 3 + + 9 9 , 3 , 6 3 I 0 L I 5 1 1 I / P L , 8 6 , 3 3 2 9 1 . 0 1 0 0 5 3 5 3 6 1 6 5 , , . 2 2 > P 2 6 > , 3 9 3 2 J , : , 1 I , : , 2 (Bourdieu ... know. F B h U \ X † « † * + , 1 , 2 ‰ Š ‹ Œ  Ž  ® ­ ¯  Ž 2 9 › P Œ 3 ’ 0 6 5 , > : 9 1 3 + , 2 , / 9 5 > ± ` Š  Œ “ Œ  Ž Ž • 2 3 6 9 5 Š ™ ‹ µ Š ‘ ± Š • Œ ’ Š ‹ “ ›  , ´ š ² . Š ‘ Š ¬ 3 Ž Œ  – I Š ° Ž ˆ . ² Š  Œ ± ² Š  Œ “ Œ  Š ³ Ž  ‹ ­ š  ‹ Š œ • › Š  Š •  ‹  Œ “ ž   Š Ÿ ( ¡ ¢ £ (  ¤ ¥ Ÿ  ¦ § ¨    ! " Ÿ )  (  ©  / 9 5 3 , , 5 7 6 1 5 + , 3 1 O 6 , 3 2 . I + 3 3 2 + L 9 6 , 6 5 3 5 9 : 9 . 1 3 3 < 0 3 q 9 5 9 5 < , * 1 , , I > 0 . 3 / 6 0 3 + P 6 9 5 , , 5 3 3 3 „ M O * + + , 6 , , 1 2 > 5 / ` 0 9 1 . / / , 9 , 2 . 6 3 6 5 0 6 9 5 6 9 5 ? O 5 < 1 9 , P 3 3 0 6 + 0 J 0 0 0 5 2 5 > 3 “ ‚  > 6 0 . / 5 9 , 9 > 7 y > , 9 > O 6 3 1 . 1 1 n 9 9 : . ,   Ÿ 6 I 2 5 : 3 + J , 3 1 + , , 2 6 L 9 5 1 , > > , . 5 / 3 0 2 3 6 0 9 1 5 , 0 I s 2 O L Ÿ s 5 r m 9 3 q 2 3 +    ª * ( M y 9 > 2 8 6 9 5 9 : . ? 0 / 3 + , 0 L L / 9 . + 1 5 6 , J / 3 6 6 5 + 0 , 3 J 2 , 3 ¶ 0 1 5 7 0 0 3 > I . , , 3 0 . M + > , 6 3 The comprehension process of audit and accounting culture? ??………… F B \ X † · † * + , 1 , 2 . I 3 2 9 P 3 0 6 5 , > : 9 1 3 + , l 1 > ` . , 2 3 6 9 5 ´ Ž ‰ Š ‹ Œ  Ž  d h U ˆ d  ® Œ Š ° Ž — › Œ ’ š Š Ž ‘ ¬ ­ ¯  Ž › Š ± ² Š  Œ “ Œ  Ž  • ‹ µ Š ™ Š ‘ ± Š • Œ ’ Š ‹ “ ² Š  Œ ± ² Š  Œ “ Œ  Š ³ Ž  ‹ ­ š  ‹ Š œ › • Š  Š •  ‹  Œ “ ž ( Š "  Ÿ (  ¡ ¢ £ Ÿ ¤  ¥ ¦ ¨   ª _ 1 , I L 0 1 0 3 9 6 : 5 9 , 2 > 2 5 2 2 L 6 9 6 9 6 6 + 5 5 3 L M 3 / P , * 9 + . 0 3 5 , , 3 3 P O , 1 + , 0 2 3 , 2 5 3 . 3 I + + 3 , , 5 / 0 1 , , 1 , , 6 / 2 2 y 5 , 9 0 3 , 6 " Ÿ ) / L < +   (  ©    Ÿ  "  Ÿ  s ? 9   J 2 J + 9 + 0 9 6 O , L 2 6 / S 5 5 9 , 6 3 , 3 5 / > 5 5 0 , 3 6 1 + / 9 , > , 3 . 3 , 5 5 > 3 + 6 0 6 3 3 , O + 0 7 1 3 . 3 2 6 + 3 5 6 + 3 1 > 2 1 2 6 5 , , , 0 + 7 , 8 6 , 3 2 3 / 9 8 0 + 5 O + , 2 > 6 I 6 0 I / 6 < 5 2 9 J 6 + I 0 O 7 1 , , 1 1 , > q 3 + , 6 5 : 9 1 < 0 3 6 9 5 5 , , > 0 3 0 O 9 1 R 6 5 J L I 0 / , M * + , 2 / 9 1 , 6 2 l m 0 5 > 6 3 / 9 7 , 1 2 r d M m 9 : 3 + , * 8 9 . * : + , N 0 I I 5 0 . / 6 / 6 O I I 1 , , . 1 0 : , B ‹ Œ  2 3 9 , 6 0 1 9 3 : 5 6 , L 0 5 2 , O 9 2 1 6 , 9 1 2 , 5 6 I 3 3 H , 0 < “  / 6 6 7 2 , . 0 / > ? ? 6 > < > + , 5 5 + . 6 > 9 1 : 6 9 0 : 5 3 J 1 < 2 Ž  \ • Ž X ¹ † Š ‹ ¸ ‹ †  Ž @ ,  6 0 2 . I 3 2 9 P 3 0 6 ‘   ¹ Ž 5 , > •  : 9 1 3 ‹ Š œ › • Š  Š • Œ “ ž _ > 7 . 0 / I 0 . 3 , 6 ( Š 2 9 2 0 5 5 : 9 1 < 3 6 5 7 , 6   9 0 6 1 P 1 8 : 5 0 5 + , 9 / , 3 + 0 3 I > * 6 6 5 0 / 5 I / 9 2 , I / 5 ` 9 O 9 2 1 , 3 7 5 / 1 , L 1 , 2 , J 6 0 7 5 3 0 J 6 , "  Ÿ : , 3 / , 5 1 + 1 ` , 6 7 , , L . , y 3 + ` Œ I 2 < 5 I . , ? 9 , / 3 2 2 / 2 . > 1 , , / 9 1 1 6 2 , > ” „ , / 8 O M 0 . , 2 3 6 5 3 , > 6 5 3 0 P , , 5 , 3 9 ¤ ? 3 ¥ ¦ 0 0 5 6 5 £ ? , 6 ¢ 5 3 2 2 > I , 9 2 5 Ž  ‹ Š • µ “ Œ  Š ‹  • Š  » ” ™  ! 2 < , , ¼ I 2 , < 3 > 5 , + , 0 : , b I 3 , , 8  1 / > “ . 7 1 ,  / ) , 0 + 2 0 3    , 3 O 3 2 2 5 Ÿ  9 + 6  ¨  " / 9 2  / 5 0 6 6 9 . 5 > 9 / , , 1 5 2 . 2 : . L , 8 Ÿ  3 9 I 6 > 3 2 / ,  m M   Ÿ  9 2 3 8 6 © 8 9 .   3 5 , 2 6 > 9 P < 6 5 2 , 3 , 0 3 6 + I , > , 0 6 / / 5 3 9 + , . 5 3 . 5 3 6 6 5 1 J M > , 8 1 9 3 . + , 0 1 answers
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Production of infectious genotype 1b virus particles in cell culture and impairment by replication enhancing mutations.

... containing the REM in NS4B release HCV particles that are infectious both in cell culture and in vivo. Our data provide an explanation for the in vitro and in vivo attenuation of cell culture adapted HCV genomes and may open new avenues for the development of fully competent culture systems covering the therapeutically most relevant HCV genotypes. Citation: ... particles, whereas an unaltered HCV genome supports production of cell culture? ??derived virus that is infectious in vitro and in vivo. Our observations provide an explanation for the attenuation of cell culture adapted HCV genomes and open new perspectives for the development of culture systems for difficult to treat HCV genotypes. increase RNA replication ... and 24 h later culture medium was harvested and filtered, whereas cells were lysed with 1% Triton X-100 in PBS. The total amount of HCV core in the cell lysate (A) and culture supernatant (B) was determined by ELISA. (C) Relative core release expressed as the fraction (in %) of total intracellular core protein that is released into the culture fluid.
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Ammonia Affects Astroglial Proliferation in Culture.

... ACCESS Citation: Bodega G, Segura B, Ciordia S, Mena MdC, López-Fernández LA, García MI, et al. (2015) Ammonia Affects Astroglial Proliferation in Culture. PLoS ONE 10(9): e0139619. doi:10.1371/journal. pone.0139619 Primary cultures of rat astroglial cells were exposed to 1, 3 and 5 mM NH4Cl for up to 10 days. Dose- and time-dependent reductions in cell numbers ... are very scarce [9, 10], but they suggest that proliferation is increased. In our work on the role of astrocytes in HE, we use astroglial cell cultures as an in vitro model. In routine monitoring of these cultures it was noticed that, at confluence, the cells continued to proliferate, but were smaller. Ammonia-treated astroglial cells, however, showed ... [11], were grown in 75 cm2 flasks (primary cultures) containing DMEM medium (Gibco) supplemented with 10% fetal bovine serum (FBS) (Gibco) and an antibiotic/antimycotic solution (Gibco), at 37°C in a 5% CO2 atmosphere. Before confluence, the cells were detached with trypsin and reseeded (forming secondary cultures) in different multiwell plates (6,
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Plant and fungal diversity in gut microbiota as revealed by molecular and culture investigations.

... Penicillium brevicompactum) were cultured on both PDA and CZAPEK media, and C. tropicalis was cultured on both Dixon agar and PDA media. Five of the cultured species of fungi (G. geotrichum, C. tropicalis, M. pachydermatis, M. globosa, and M. restricta) were also identified by clone sequencing, while 11 fungi were detected only by culture (Figure 1). Penicillium, ... Cladosporidium sp., Climacocystis sp. and C. tropicalis) were cultured on Dixon agar medium. Three species of fungi (Penicillium sp./P. commune/P. camemberti, Aspergillus versicolor, Beauveria bassiana) were cultured on Potato Dextrose media. Two species of fungi (Aspergillus flavipes, Isaria farinosa) were cultured on CZAPEK medium. Two species (Hypocrea lixii/Penicillium ... different culture media were used to isolate fungi, and these cultured fungi were further identified by ITS sequencing. A total of 37 eukaryotic species were identified, including a Diatoms (Blastocystis sp.) species, 18 plant species from the Streptophyta phylum and 18 fungal species from the Ascomycota, Basidiomycota and Chytridiocomycota phyla. Cultures
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Research on the changes of some physiological parameters in several fish species under the action of the talstar insecticide

... The Tlastar product, under the concentrations from 0.000625 to 0.005 ml/l water, produces, after one week of immersion, a significant decrease of the fish oxygen consumption. The insecticide has changed the fish respiratory rhythm in all investigated concentrations after seven days of exposure. The number of erythrocytes has significantly decrease after ... the ecotoxicological risks caused by pesticides to ecosystems is based on toxicity data and effects of pesticide preparations on nontarget organisms (like fish) [30]. Bifenthrin is highly toxic to fish and aquatic arthropods and LC50 values range from 0.0038 to17.8 μg/L and is only slightly toxic to both waterfowl and upland game birds (LD50 values ... more toxic to cold – than warm – water fish, but the toxicity of pyrethroids is little affected by pH or water hardness [13]. The aim of this study is to analyze the influence of Talstar insecticide upon some physiological parameters (oxygen consumption, breathing frequency, number of red blood cells) in freshwater fishes: prussian carp (Carassius auratus
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A study to measure the impact of organizational culture and organizational excellence

... organizational culture and quality of different strategies. 3. There is a meaningful relationship between organizational culture and quality of human resources. 4. There is a meaningful relationship between organizational culture and quality of participating in organizational resources. 5. There is a meaningful relationship between organizational culture ... 1996). Organizational culture plays an important role on increasing organization excellence and there are many evidences through different studies on this relationship. MacIntosh and Doherty (2010) investigated the effect of organizational culture on job satisfaction and decision to leave the organization by studying fitness staff. Organizational culture is commonly ... organizational culture, which could promote employee fulfillment and satisfaction and reported that they were positively associated with leadership and personal effectiveness. According to their survey, the perceived relationship across instances was stronger between organizational culture and leadership effectiveness than between organizational culture and
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Effect of Somatic Cell Types and Culture Medium on in vitro Maturation, Fertilization and Early Development Capability of Buffalo Oocytes

... Tyrode+BOEC. Fertilized oocytes were cultured for early embryonic development in TCM-199 with and without BOEC. Higher maturation rates were observed in the granulosa (84.24%) and cumulus cells (83.44%) than BOEC co culture system (73.37%). Highest fertilization rate was obtained in modified Ca++ free Tyrode with BOEC co culture (70.42%), followed by modified ... inseminated oocytes were transferred to 200 µl of TCM-199 supplemented with BOEC. A control culture lacking BOEC was established. Only oocytes fertilized in Ca++ free tyrode medium were cultured in TCM-199 at 39ºC in 5%CO2 in humidified air for early development. The culture medium was changed every two days and embryo development up was recorded. Statistical ... Accessible at: www.pvj.com.pk Effect of Somatic Cell Types and Culture Medium on in vitro Maturation, Fertilization and Early Development Capability of Buffalo Oocytes H. Jamil*, H. A. Samad, N. Rehman, Z. I. Qureshi and L. A. Lodhi Department of Theriogenology, University of Agriculture, Faisalabad, Pakistan *Corresponding author: drhjamil@hotmail.com ARTICLE
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9. znanstvena konferenca Globalizacija kulture = 9th Scientific Conference "Globalisation of Culture"

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ESTABILIDADE QUÍMICA E MICROBIOLÓGICA DE "MINCED FISH" DE PEIXES AMAZÔNICOS DURANTE O CONGELAMENTO.

... microbiológicos. Brasília: LANARA, 1981. 112p. [2] FAO/WHO Draft Revised Standard for Quick Frozen Blocks of Fish Fillets, Minced Fish Flesh and Mixtures of Fillets and Minced Fish Flesh (Appendix IV). In: Report of the 21st Session of the Codex Committee on Fish and Fishery Products. Rome: Codex Alimentarius Commission, 1994. p. 47-57. Ciênc. Tecnol. Aliment., ... “minced fish? ?? e/ou o “surimi” como seu principal ingrediente. Os produtos de “minced fish? ?? são de uma geração mais antiga que aqueles à base de “surimi”, e normalmente são vistos como tendo seu lugar ocupado cada vez mais por estes últimos, no entanto o seu processo de produção é relativamente simples e mais amplamente aplicável. O “minced fish? ?? é ... “minced fish? ?? estocados a -18±1°C e -36±1°C mostraram pouca variação ao longo dos 150 dias de estocagem, ficando entre 6,50 e 7,07. Os “minced fish? ?? de mapará e pacú apresentaram valores de pH superiores a 6,75 no início do experimento. Na temperatura de -36°C, todas as amostras apresentaram aumento do valor de pH ao longo do tempo. A -18°C, os “minced fish? ??
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Comparison of microbiological culture and polymerase chain reaction in the diagnosis of salmonellosis in Salmonella Typhimurium-infected calves.

... Typhimurium ABSTRACT The efficiency of microbiological culture and polymerase chain reaction (PCR) for detection of Salmonella Typhimurium is compared in fecal samples of Holstein calves experimentally infected with 109 CFU of Salmonella Typhimurium. Seventy-two fecal samples were analyzed by microbiological culture and PCR associated with selenite cystine ... enrichment broths. Regardless of the selective enrichment broth, the microbiological culture was significantly better than PCR for detection of positive samples of Salmonella Typhimurium. The selective enrichment broths SC and TMK had no effect on the efficiency of the microbiological culture. The SC broth was the best option as selective enrichment associated ... microbiológico e da reação em cadeia da polimerase no diagnóstico de salmonelose em bezerros infectados experimentalmente com Salmonella Typhimurium [Comparison of microbiological culture and polymerase chain reaction in the diagnosis of salmonellosis in Salmonella Typhimurium-infected calves] D.G. Silva1, L.G. Ávila2, R. Berg3, D.R. Silva1, S.O. Conde4,
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Culture sites in the diagnosis of gonorrhea in women

... Of the patients with overgrown cultures, 14 had all three cultures involved. The rectal specimens were the ones most commonly overgrown, corresponding to all but four of the patients with such cultures. Overgrowth of either the endocervical or urethral culture occurred only in one patient: the endocervical and urethral cultures were both reported overgrown ... approached which are the best sites to culture individually but rather by finding ways to combine specimens on the same culture plate so that more sites can be examined without additional cost. The combination of culture sites on one agar plate could conceivably increase “overIn the study, 14 patients had growth.” cultures that were completely overgrown when ... U.S.A. 4PublicHealth Statistician, AlamedaCounty Health Care Services Agency, Oakland, California, U.S.A. Although the culture medium and conditions of incubation of inoculated cultures are extremely important for isolation of the agent, a culture? ??s overall efficiency (defined is as maximum recovery of N. gonorrhoeae) still directly related to the adequacy
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An improved outfit for the isolation of brucella, salmonella, etc. by blood culture

... AN IMPROVED OUTFIT SALMONELLA, DR. FAO/WHO FOR THE ISOLATION OF BRUCELLA, ETC. BY BLOOD CULTURE* M. RUIZ Brucellosis CASTAREDA Centre, The method developed by the author for cultivation of blood in brucellosis (1, 2) requires a layer of agar medium placed on the side ... the side of a bottle while a certain amount of broth remains at the bottom of the same bottle. The usefulness of this double medium relies on the facility of detection of positive cultures without cumbersome transfers from liquid to solid media. From those who have adopted this method some use rectangular bottles, others use cylindrical bottles but in al1 ... Media prepared in this outfit may be safely mailed with no loss in the shape of the slant. A further advantage of this outfit consists in the possibility of referring positive cultures to specialized laboratories by the simple procedure of removing aseptically the mixture of broth and blood, replacing the stoppers and sending the bottle through the mail. Instructions
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